Difference between revisions of "PMID:6808961"
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*Reference Condition: | *Reference Condition: | ||
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| − | + | OMP:0006040 | |
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| − | + | decreased resistance to quinolone | |
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| − | 000034 | + | ECO:000034 |
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non-traceable author statement | non-traceable author statement | ||
Revision as of 18:21, 16 June 2014
| Citation |
Brunner, DP , Graham, GS and Treick, RW (1982) Exchange of colicin receptor capacity between strains of Escherichia coli sensitive or resistant to colicin K-K235. Arch. Microbiol. 131:229-34 |
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| Abstract |
Phage and colicin-resistant mutants were derived from Escherichia coli K-12P678. Two classes of phage T6 and colicin K-resistant mutants (genotype tsx) were isolated. Tsx-2 mutants, which demonstrated mucoid growth and increased sensitivities to many antibiotics, became sensitive to colicin K when pretreated with ethylenediaminetetraacetate (EDTA), whereas Tsx-1 mutants did not. Reassociation of EDTA-released material partially restored resistance to colicin K for Tsx-2 mutants. When EDTA-released material from strain P678 was associated with either class of K-resistant mutant, an increase in colicin K sensitivity resulted. Observations suggest that colicin K can act on its target site once it penetrates the cell surface. In addition, results suggest that functional colicin K receptors can be transferred from sensitive to resistant strains, thus conferring colicin sensitivity. |
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| Keywords |
Cell Membrane/metabolism; Colicins/metabolism; Colicins/pharmacology; Coliphages/genetics; Drug Resistance, Microbial; Edetic Acid/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Kinetics; Membrane Proteins/isolation & purification; Receptors, Cell Surface; Receptors, Immunologic/genetics; Receptors, Immunologic/metabolism |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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a mutation or genetic difference within a strain |
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0000181 |
in vitro assay evidence |
The tsx-2 class mutants were resistant toward the Phage T6. |
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a mutation or genetic difference within a strain |
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OMP:0006049 |
increased resistance to bacteriocin |
ECO:0000181 |
in vitro assay evidence |
The tsx-2 class mutants were resistant toward the Colicin K. |
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a mutation or genetic difference within a strain |
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0000181 |
in vitro assay evidence |
The tsx-1 class of mutants contained a slight resistances toward phage T6 |
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a mutation or genetic difference within a strain |
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OMP:0006049 |
increased resistance to bacteriocin |
ECO:0000181 |
in vitro assay evidence |
The tsx-1 class of mutants contained a slight resistances toward Colicin K. |
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a mutation or genetic difference within a strain |
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0000181 |
in vitro assay evidence |
It was found that the class tsx-2 mutants produced mucoid growth at temperatures 30oC and 370C. This was believed to be due to the loss of protien a as well as various other membrane proteins(fig. 1). |
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a mutation or genetic difference within a strain |
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OMP:0006057 |
decreased resistance to aminoglycoside |
ECO:000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotic kanamycin. |
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a mutation or genetic difference within a strain |
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OMP:0000276 |
increased resistance to antimicrobial compound |
000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotics ampicillin and carbenicillin. |
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a mutation or genetic difference within a strain |
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OMP:0000276 |
increased resistance to antimicrobial compound |
000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotic chloramphenicol. |
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a mutation or genetic difference within a strain |
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OMP:0000276 |
increased resistance to antimicrobial compound |
000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotic nalidixic acid. |
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a mutation or genetic difference within a strain |
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OMP:0000276 |
increased resistance to antimicrobial compound |
000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotic novobiocin. |
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a mutation or genetic difference within a strain |
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OMP:0006040 |
decreased resistance to quinolone |
ECO:000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotic nalidixic acid. |
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a mutation or genetic difference within a strain |
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|
OMP:0000276 |
increased resistance to antimicrobial compound |
ECO:000034 |
non-traceable author statement |
The tsx-2 mutation caused increased sensitivity toward the antibiotics neomycin. |
|
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</protect>
Notes
References
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