Difference between revisions of "PMID:5327656"
(Table edited by Azweifel via TableEdit) |
(Table edited by Azweifel via TableEdit) |
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| Line 67: | Line 67: | ||
''lacI<sup>S</sup>'' | ''lacI<sup>S</sup>'' | ||
| | | | ||
| − | + | *Abolished utilization of carbon source (lactose) | |
| + | *Regulation of lac operon expression *Constitutive repression of lac operon expression) | ||
| | | | ||
Expression | Expression | ||
| | | | ||
| − | dominant over ''i<sup>+</sup>'' and not inducible by lactose | + | *dominant over ''i<sup>+</sup>'' and not inducible by lactose |
| + | *unable to utilize lactose | ||
| | | | ||
| | | | ||
| − | + | Complementation | |
|- | |- | ||
| | | | ||
| Line 86: | Line 88: | ||
''lacI<sup>S</sup>'' | ''lacI<sup>S</sup>'' | ||
| | | | ||
| − | + | *Positive suppression | |
| + | *Utilization of carbon source (lactose) | ||
| | | | ||
Expression | Expression | ||
| Line 94: | Line 97: | ||
| | | | ||
| − | + | Suppression | |
|- | |- | ||
| | | | ||
| Line 105: | Line 108: | ||
''lacO<sup>C</sup>'' | ''lacO<sup>C</sup>'' | ||
| | | | ||
| − | + | * Positive suppression | |
| + | * Utilization of carbon source (lactose) | ||
| | | | ||
Expression | Expression | ||
| | | | ||
| − | 125 ''lac<sup>+</sup>'' revertants | + | 125 ''lac<sup>+</sup>'' revertants are now able to utilize lactose |
| | | | ||
| Line 124: | Line 128: | ||
''i<sup>S</sup>i<sup>-</sup>'' | ''i<sup>S</sup>i<sup>-</sup>'' | ||
| | | | ||
| − | + | *Abolished utilization of carbon source (lactose) | |
| + | *Regulation of lac operon expression *Constitutive repression of lac operon expression) | ||
| | | | ||
Expression | Expression | ||
| Line 143: | Line 148: | ||
''i<sup>S</sup>i<sup>-</sup>'' | ''i<sup>S</sup>i<sup>-</sup>'' | ||
| | | | ||
| − | + | *Abolished utilization of carbon source (lactose) | |
| + | *Regulation of lac operon expression *Constitutive repression of lac operon expression) | ||
| | | | ||
Expression | Expression | ||
| Line 162: | Line 168: | ||
''i<sup>S</sup>i<sup>-774</sup>'' | ''i<sup>S</sup>i<sup>-774</sup>'' | ||
| | | | ||
| − | + | *Inducible expression of beta-galactosidase | |
| + | |||
| | | | ||
Expression | Expression | ||
Revision as of 14:37, 26 January 2011
| Citation |
Bourgeois, S, Cohn, M and Orgel, LE (1965) Suppression of and complementation among mutants of the regulatory gene of the lactose operon of Escherichia coli.J. Mol. Biol. 14:300-2 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Escherichia coli; Genes; Genetic Code; Lactose; Molecular Biology; Mutation |
| edit table |
Main Points of the Paper
- The regulatory gene, i, codes for a protein whereas the operator gene, o, probably does not
Materials and Methods Used
- Parent Strain: Δlac su-/ FiSo+z+y+
- iS- dominant over i+ and not inducible by lactose sz 43
- 5 strains with suppressor mutations
- su-
- su+1
- su+2
- su+3
- su+4
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSo+z+y+ |
lacIS |
|
Expression |
|
Complementation | |
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSi-o+z+y+ |
lacIS |
|
Expression |
975 lac+ revertants |
Suppression | |
|
Escherichia coli |
NCBI:562 |
Δlac su- /FiSoCo+z+y+ |
lacOC |
|
Expression |
125 lac+ revertants are now able to utilize lactose |
||
|
Escherichia coli |
NCBI:562 |
lac+ su+1 /FiSi-o+z+y+ |
iSi- |
|
Expression |
restore the iS phenotype to 10 revertants- giving rise to dominant lac- diploids in i+su+1 |
Plating Assay |
in text, pg. 300 |
|
Escherichia coli |
NCBI:562 |
lac+ su+ /FiSi-o+z+y+ |
iSi- |
|
Expression |
restore the iS phenotype in 14 revertants- giving rise to dominant lac- diploids in i+su+1 or su+3 |
Plating Assay |
in text, pg. 300-301 |
|
Escherichia coli |
NCBI:562 |
lac+ su+3 /FiSi-774o+z+y+ |
iSi-774 |
|
Expression |
iSi-774 repressor, when suppressed by su+3, becomes inducible |
Biochemical Assay |
Table 2- beta-galactosidase assay |
|
Escherichia coli |
NCBI:562 |
lac+ su+ /FiSi-o+z+y+ |
iSi-1058 |
unable to utilize lactose |
Expression |
dramatically suppressed by both su+1 and su+3 |
Biochemical Assay |
Table 2- beta-galactosidase assay |
| edit table |
</protect>
