Difference between revisions of "PMID:4933690"
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''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
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| − | + | *Decreased ability to maintain concentration gradient? | |
| + | *Abolished energy-coupling transporter activity? | ||
| + | *Absent intracellular accumulation of thiomethylgalactoside (direct observation) | ||
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Other | Other | ||
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''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
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| − | + | *Decreased intracellular accumulation of substance | |
| + | *Increased rate of transporter efflux? | ||
| + | *Decreased energy-coupled transport? | ||
| + | *Increased rate of efflux of thiomethylgalactoside (TMG) (direct observation) | ||
| | | | ||
Other | Other | ||
| Line 106: | Line 111: | ||
''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
| | | | ||
| − | + | *Increased rate of transporter substrate uptake? | |
| + | *Increased transport of small molecules | ||
| + | *Increased rate of transporter activity | ||
| | | | ||
Metabolic Activity | Metabolic Activity | ||
Revision as of 11:38, 27 January 2011
| Citation |
Wilson, TH, Kusch, M and Kashket, ER (1970) A mutant in Escherichia coli energy-uncoupled for lactose transport: a defect in the lactose-operon.Biochem. Biophys. Res. Commun. 40:1409-14 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport, Active; Carbon Isotopes; Cell Membrane; Coliphages; Escherichia coli; Galactose; Glycosides; Lactose; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic |
| edit table |
Main Points of the Paper
- Isolation of mutant (X71-54) with a severe defect in TMG accumulation
- possesses elevated membrane carrier activity, therefore the rate of efflux is increased
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-, F-)
- Additional Strain: X71-54
- Additional Strain: Ca77 (lactose-deleted, B1-, Hfr)
- β-galactosidase assay
- ONPG uptake assay
- Thiomethylgalactoside (TMG) transport assay
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
|
Other |
Intracellular [14C]TMG accumulation |
Other |
Figure 1- Filter assay |
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
|
Other |
exit rate of [14C]TMG was faster than that found for the parent |
Other |
Figure 2- Filter assay |
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
|
Metabolic Activity |
130% of normal uptake activity (gauged by ONPG entry) |
Biochemical Assay |
Table 1- ONPG assay |
| edit table |
</protect>
Notes
Additional papers regarding this mutant can be found here
