Difference between revisions of "PMID:19187760"

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'''Madabhushi, R and Marians, KJ'''  (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.''Mol. Cell'' '''33''':171-80
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!align=left  |Abstract
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In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19187760 PubMed]
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Online version:[http://dx.doi.org/10.1016/j.molcel.2009.01.001 10.1016/j.molcel.2009.01.001]
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!align=left  |Keywords
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Actins; Binding Sites; Cell Cycle; Chromosome Segregation; Chromosomes, Bacterial; Cytoskeleton; DNA Topoisomerase IV; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Biological; Mutation
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes
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==Notes==
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==References==
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<references/>

Revision as of 13:37, 14 March 2011

Citation

Madabhushi, R and Marians, KJ (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.Mol. Cell 33:171-80

Abstract

In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation.

Links

PubMed Online version:10.1016/j.molcel.2009.01.001

Keywords

Actins; Binding Sites; Cell Cycle; Chromosome Segregation; Chromosomes, Bacterial; Cytoskeleton; DNA Topoisomerase IV; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Biological; Mutation

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

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Notes

References

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