Difference between revisions of "PMID:19187760"
(→Main Points of the Paper) |
(→Materials and Methods Used) |
||
| Line 37: | Line 37: | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
| − | + | *Fluorescence microscopy | |
| + | * | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 13:48, 14 March 2011
| Citation |
Madabhushi, R and Marians, KJ (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli.Mol. Cell 33:171-80 |
|---|---|
| Abstract |
In Escherichia coli, topoisomerase IV, a type II topoisomerase, mediates the resolution of topological linkages between replicated daughter chromosomes and is essential for chromosome segregation. Topo IV activity is restricted to only a short interval late in the cell cycle. However, the mechanism that confers this temporal regulation is unknown. Here we report that the bacterial actin homolog MreB participates in the temporal oscillation of Topo IV activity. We show that mreB mutant strains are deficient in Topo IV activity. In addition, we demonstrate that, depending upon whether it is in a monomeric or polymerized state, MreB affects Topo IV activity differentially. In addition, MreB physically interacts with the ParC subunit of Topo IV. Together, these results may explain how dynamics of the bacterial cytoskeleton are coordinated with the timing of chromosome segregation. |
| Links |
PubMed Online version:10.1016/j.molcel.2009.01.001 |
| Keywords |
Actins; Binding Sites; Cell Cycle; Chromosome Segregation; Chromosomes, Bacterial; Cytoskeleton; DNA Topoisomerase IV; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Models, Biological; Mutation |
| edit table |
Main Points of the Paper
- MreB and TopoIV physically and functionally interact
Materials and Methods Used
- Fluorescence microscopy
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
PA340-678 |
mreB |
increased size of nucleoid |
Morphology |
Microscopy |
Figure 1 and 2 | ||
|
Escherichia coli |
PA340-678 |
mreB |
decreased topoisomerase IV activity |
Morphology |
Microscopy |
Figure 1 and 2 | ||
|
Escherichia coli |
NCBI:83333 |
PA340 |
dumbbell-shaped nucleoids |
Morphology |
Microscopy |
Figure 1 and 2 | ||
|
Escherichia coli |
NCBI:83333 |
PA340-678 |
mreB |
increased number of singlet nucleoids |
Morphology |
Microscopy |
Figure 1 and 2 | |
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
