Difference between revisions of "PMID:25848053"

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'''Lee, SA, Gallagher, LA, Thongdee, M, Staudinger, BJ, Lippman, S, Singh, PK and Manoil, C'''  (2015) General and condition-specific essential functions of Pseudomonas aeruginosa. ''Proc. Natl. Acad. Sci. U.S.A.'' '''112''':5189-94
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!align=left align='left' bgcolor='#CCCCFF' |Abstract
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The essential functions of a bacterial pathogen reflect the most basic processes required for its viability and growth, and represent potential therapeutic targets. Most screens for essential genes have assayed a single condition--growth in a rich undefined medium--and thus have not distinguished genes that are generally essential from those that are specific to this particular condition. To help define these classes for Pseudomonas aeruginosa, we identified genes required for growth on six different media, including a medium made from cystic fibrosis patient sputum. The analysis used the Tn-seq circle method to achieve high genome coverage and analyzed more than 1,000,000 unique insertion positions (an average of one insertion every 6.0 bp). We identified 352 general and 199 condition-specific essential genes. A subset of assignments was verified in individual strains with regulated expression alleles. The profile of essential genes revealed that, compared with Escherichia coli, P. aeruginosa is highly vulnerable to mutations disrupting central carbon-energy metabolism and reactive oxygen defenses. These vulnerabilities may arise from the stripped-down architecture of the organism's carbohydrate utilization pathways and its reliance on respiration for energy generation. The essential function profile thus provides fundamental insights into P. aeruginosa physiology as well as identifying candidate targets for new antibacterial agents.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=25848053 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4413342 PMC4413342]
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Online version:[http://dx.doi.org/10.1073/pnas.1422186112 10.1073/pnas.1422186112]
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Carbon/metabolism; DNA Transposable Elements/genetics; Escherichia coli/genetics; Genes, Bacterial; Genes, Essential; Mutagenesis, Insertional/genetics; Phylogeny; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development; Pseudomonas aeruginosa/metabolism
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 12:07, 8 July 2015

Citation

Lee, SA, Gallagher, LA, Thongdee, M, Staudinger, BJ, Lippman, S, Singh, PK and Manoil, C (2015) General and condition-specific essential functions of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. U.S.A. 112:5189-94

Abstract

The essential functions of a bacterial pathogen reflect the most basic processes required for its viability and growth, and represent potential therapeutic targets. Most screens for essential genes have assayed a single condition--growth in a rich undefined medium--and thus have not distinguished genes that are generally essential from those that are specific to this particular condition. To help define these classes for Pseudomonas aeruginosa, we identified genes required for growth on six different media, including a medium made from cystic fibrosis patient sputum. The analysis used the Tn-seq circle method to achieve high genome coverage and analyzed more than 1,000,000 unique insertion positions (an average of one insertion every 6.0 bp). We identified 352 general and 199 condition-specific essential genes. A subset of assignments was verified in individual strains with regulated expression alleles. The profile of essential genes revealed that, compared with Escherichia coli, P. aeruginosa is highly vulnerable to mutations disrupting central carbon-energy metabolism and reactive oxygen defenses. These vulnerabilities may arise from the stripped-down architecture of the organism's carbohydrate utilization pathways and its reliance on respiration for energy generation. The essential function profile thus provides fundamental insights into P. aeruginosa physiology as well as identifying candidate targets for new antibacterial agents.

Links

PubMed PMC4413342 Online version:10.1073/pnas.1422186112

Keywords

Carbon/metabolism; DNA Transposable Elements/genetics; Escherichia coli/genetics; Genes, Bacterial; Genes, Essential; Mutagenesis, Insertional/genetics; Phylogeny; Pseudomonas aeruginosa/genetics; Pseudomonas aeruginosa/growth & development; Pseudomonas aeruginosa/metabolism

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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