Difference between revisions of "PMID:1100857"

Revision as of 16:03, 26 October 2010

Citation	Aswad, D and Koshland, DE (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.J. Mol. Biol. 97:225-35
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Species Specificity
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Please summarize the main points of the paper.

Isolation of non-chemotactic mutants
Description of the preformed liquid gradient technique
- utilizes a preformed gradient in a vertical column of liquid medium
- separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
- 68 exhibited smooth co-ordinated motility with no evidence of tumbling
- 4 exhibited uncoordinated motility
Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
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@@ Line 35: / Line 35: @@
 ==Main Points of the Paper ==
 {{LitSignificance}}
+* Isolation of non-chemotactic mutants
+* Description of the preformed liquid gradient technique
+**utilizes a preformed gradient in a vertical column of liquid medium
+**separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
+* None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
+**68 exhibited smooth co-ordinated motility with no evidence of tumbling
+**4 exhibited uncoordinated motility
+* Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella
 == Materials and Methods Used ==