Difference between revisions of "PMID:27573014"
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| + | !align=left align='left' bgcolor='#CCCCFF' |Citation | ||
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| + | '''Jorgenson, MA and Young, KD''' (2016) Interrupting biosynthesis of O-antigen or the lipopolysaccharide core produces morphological defects in Escherichia coli by sequestering undecaprenyl phosphate. ''J. Bacteriol.'' '''''' | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Abstract | ||
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| + | Undecaprenyl phosphate (Und-P) is a member of the family of essential polyprenyl phosphate lipid carriers and, in the Gram-negative bacterium Escherichia coli, is required for synthesizing the peptidoglycan (PG) cell wall, enterobacterial common antigen (ECA), O-antigen and colanic acid. Previously, we found that interrupting ECA biosynthesis indirectly alters PG synthesis by sequestering Und-P via dead-end intermediates, causing morphological defects. To determine if competition for Und-P was a more general phenomenon, we determined if O-antigen intermediates caused similar effects. Indeed, disrupting the synthesis of O-antigen or LPS core oligosaccharide induced cell shape deformities, which were suppressed by preventing the initiation of O-antigen biosynthesis or by manipulating Und-P metabolism. We conclude that accumulating O-antigen intermediates alters PG synthesis by sequestering Und-P. Importantly, many previous experiments addressed the physiological functions of various oligosaccharides and glyconjugates, but these studies employed mutants that accumulate deleterious intermediates. Thus, conclusions based on these experiments must be re-evaluated to account for possible indirect effects of Und-P sequestration. | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=27573014 PubMed] | ||
| + | Online version:[http://dx.doi.org/10.1128/JB.00550-16 10.1128/JB.00550-16] | ||
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| + | !align=left align='left' bgcolor='#CCCCFF' |Keywords | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Latest revision as of 12:24, 19 October 2016
| Citation |
'Jorgenson, MA and Young, KD (2016) Interrupting biosynthesis of O-antigen or the lipopolysaccharide core produces morphological defects in Escherichia coli by sequestering undecaprenyl phosphate. J. Bacteriol. ' |
|---|---|
| Abstract |
Undecaprenyl phosphate (Und-P) is a member of the family of essential polyprenyl phosphate lipid carriers and, in the Gram-negative bacterium Escherichia coli, is required for synthesizing the peptidoglycan (PG) cell wall, enterobacterial common antigen (ECA), O-antigen and colanic acid. Previously, we found that interrupting ECA biosynthesis indirectly alters PG synthesis by sequestering Und-P via dead-end intermediates, causing morphological defects. To determine if competition for Und-P was a more general phenomenon, we determined if O-antigen intermediates caused similar effects. Indeed, disrupting the synthesis of O-antigen or LPS core oligosaccharide induced cell shape deformities, which were suppressed by preventing the initiation of O-antigen biosynthesis or by manipulating Und-P metabolism. We conclude that accumulating O-antigen intermediates alters PG synthesis by sequestering Und-P. Importantly, many previous experiments addressed the physiological functions of various oligosaccharides and glyconjugates, but these studies employed mutants that accumulate deleterious intermediates. Thus, conclusions based on these experiments must be re-evaluated to account for possible indirect effects of Und-P sequestration. |
| Links |
PubMed Online version:10.1128/JB.00550-16 |
| Keywords |
|
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
