Difference between revisions of "PMID:21212055"
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==Phenotype Annotations== | ==Phenotype Annotations== | ||
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*Taxon: Escherichia coli | *Taxon: Escherichia coli | ||
*Strain: K-12 | *Strain: K-12 | ||
| + | *Substrain: ME12 | ||
| + | *NCBI Taxon ID: 8333 | ||
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*Genotype of Reference Strain: ''recA'' | *Genotype of Reference Strain: ''recA'' | ||
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| − | + | Once the ''recA'' gene was deactivated various antibiotics were added at sublethal levels. This resulted in a decrease in the mutant frequency compared to the wildtype, especially with the antibiotics Gentamicin, Rifampicin and Chloramphenical. | |
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Revision as of 15:17, 9 December 2011
| Citation |
Thi, TD, López, E, Rodríguez-Rojas, A, Rodríguez-Beltrán, J, Couce, A, Guelfo, JR, Castañeda-García, A and Blázquez, J (2011) Effect of recA inactivation on mutagenesis of Escherichia coli exposed to sublethal concentrations of antimicrobials.J. Antimicrob. Chemother. 66:531-8 |
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| Abstract |
Low concentrations of some antibiotics have been reported to stimulate mutagenesis and recombination, which may facilitate bacterial adaptation to different types of stress, including antibiotic pressure. However, the mutagenic effect of most of the currently used antibiotics remains untested. Furthermore, it is known that in many bacteria, including Escherichia coli, stimulation of mutagenesis is mediated by the SOS response. Thus, blockage or attenuation of this response through the inhibition of RecA has been proposed as a possible therapeutic adjuvant in combined therapy to reduce the ability to generate antibiotic-resistant mutants. The aim of this work was to study the capacity of sublethal concentrations of antimicrobials of different families with different molecular targets to increase the mutant frequency of E. coli, and the effect that inactivation of recA would have on antibiotic-mediated mutagenesis. |
| Links |
PubMed Online version:10.1093/jac/dkq496 |
| Keywords |
Anti-Bacterial Agents; Escherichia coli; Gene Deletion; Humans; Mutagenesis; Mutagens; Mutation; Rec A Recombinases |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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a mutation or genetic difference within a strain |
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Once the recA gene was deactivated various antibiotics were added at sublethal levels. This resulted in a decrease in the mutant frequency compared to the wildtype, especially with the antibiotics Gentamicin, Rifampicin and Chloramphenical. |
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Notes
References
See Help:References for how to manage references in omp dev.
