Difference between revisions of "PMID:4566452"
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| + | {| id="C4eee3b4757825" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Weber, K, Platt, T, Ganem, D and Miller, JH''' (1972) Altered sequences changing the operator-binding properties of the Lac repressor: colinearity of the repressor protein with the i-gene map.''Proc. Natl. Acad. Sci. U.S.A.'' '''69''':3624-8 | ||
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| + | !align=left |Abstract | ||
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| + | A technique is described for mapping point mutations in the first 59 amino-acid residues of the lac repressor from Escherichia coli, using less than 0.1 mumol (4 mg) of the purified protein. This technique was used to localize five mutations affecting the ability of the i-gene product to repress in vivo. These alterations are located at four different sites in the amino-terminal region of the repressor molecule. Three of these are missense mutations and result in changes from serine to proline (residue 16), threonine to alanine (residue 19), and alanine to valine (residue 53). Each amino-acid substitution alone is sufficient to eliminate repression in vivo, presumably by altering the operator binding activity. The remaining two independently-isolated mutations are identical, and result in a change from a glutamine codon at position 26 to an amber (UAG) codon. Since suppression of this nonsense mutation with amber suppressors that insert leucine, tyrosine, serine, or glutamine restores repressor activity to the molecule, glutamine(26) cannot be crucial for the operator-binding function. A comparison of the position of each altered residue with the genetic map enabled us to estimate the physical distance between several deletion-group endpoints. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4566452 PubMed] | ||
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| + | !align=left |Keywords | ||
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| + | Amino Acid Sequence; Amino Acids; Chromatography; Chromatography, Gel; Chromosome Mapping; Chymotrypsin; Electrophoresis, Paper; Escherichia coli; Galactosidases; Genes, Regulator; Lactose; Mutation; Operon; Peptides; Protein Binding; Protein Conformation | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
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| + | {| id="H4eee3b47a7d77" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
| + | |||
| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | |||
| + | [[Category:Publication]] | ||
Latest revision as of 14:13, 18 December 2011
| Citation |
Weber, K, Platt, T, Ganem, D and Miller, JH (1972) Altered sequences changing the operator-binding properties of the Lac repressor: colinearity of the repressor protein with the i-gene map.Proc. Natl. Acad. Sci. U.S.A. 69:3624-8 |
|---|---|
| Abstract |
A technique is described for mapping point mutations in the first 59 amino-acid residues of the lac repressor from Escherichia coli, using less than 0.1 mumol (4 mg) of the purified protein. This technique was used to localize five mutations affecting the ability of the i-gene product to repress in vivo. These alterations are located at four different sites in the amino-terminal region of the repressor molecule. Three of these are missense mutations and result in changes from serine to proline (residue 16), threonine to alanine (residue 19), and alanine to valine (residue 53). Each amino-acid substitution alone is sufficient to eliminate repression in vivo, presumably by altering the operator binding activity. The remaining two independently-isolated mutations are identical, and result in a change from a glutamine codon at position 26 to an amber (UAG) codon. Since suppression of this nonsense mutation with amber suppressors that insert leucine, tyrosine, serine, or glutamine restores repressor activity to the molecule, glutamine(26) cannot be crucial for the operator-binding function. A comparison of the position of each altered residue with the genetic map enabled us to estimate the physical distance between several deletion-group endpoints. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acids; Chromatography; Chromatography, Gel; Chromosome Mapping; Chymotrypsin; Electrophoresis, Paper; Escherichia coli; Galactosidases; Genes, Regulator; Lactose; Mutation; Operon; Peptides; Protein Binding; Protein Conformation |
| edit table |
Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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