Difference between revisions of "PMID:18532981"

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'''Zhang, X and Newman, E'''  (2008) Deficiency in l-serine deaminase results in abnormal growth and cell division of Escherichia coli K-12.''Mol. Microbiol.'' '''69''':870-81
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!align=left  |Abstract
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The loss of the ability to deaminate l-serine severely impairs growth and cell division in Escherichia coli K-12. A strain from which the three genes (sdaA, sdaB, tdcG) coding for this organism's three l-serine deaminases had been deleted grows well in glucose minimal medium but, on subculture into minimal medium with glucose and casamino acids, it makes very large, abnormally shaped cells, many of which lyse. When inoculated into Luria-Bertani (LB) broth with or without glucose, it makes very long filaments. Provision of S-adenosylmethionine restores cell division in LB broth with glucose, and repairs much of the difficulty in growth in medium with casamino acids. We suggest that replication of E. coli is regulated by methylation, that an unusually high intracellular l-serine concentration, in the presence of other amino acids, starves the cell for S-adenosylmethionine and that it is the absence of S-adenosylmethionine and/or of C1-tetrahydrofolate derivatives that prevents normal cell division.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=18532981 PubMed]
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Online version:[http://dx.doi.org/10.1111/j.1365-2958.2008.06315.x 10.1111/j.1365-2958.2008.06315.x]
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!align=left  |Keywords
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Amino Acids; Cell Division; Culture Media; Escherichia coli; Escherichia coli Proteins; Gene Deletion; L-Serine Dehydratase; S-Adenosylmethionine
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 20:38, 20 December 2011

Citation

Zhang, X and Newman, E (2008) Deficiency in l-serine deaminase results in abnormal growth and cell division of Escherichia coli K-12.Mol. Microbiol. 69:870-81

Abstract

The loss of the ability to deaminate l-serine severely impairs growth and cell division in Escherichia coli K-12. A strain from which the three genes (sdaA, sdaB, tdcG) coding for this organism's three l-serine deaminases had been deleted grows well in glucose minimal medium but, on subculture into minimal medium with glucose and casamino acids, it makes very large, abnormally shaped cells, many of which lyse. When inoculated into Luria-Bertani (LB) broth with or without glucose, it makes very long filaments. Provision of S-adenosylmethionine restores cell division in LB broth with glucose, and repairs much of the difficulty in growth in medium with casamino acids. We suggest that replication of E. coli is regulated by methylation, that an unusually high intracellular l-serine concentration, in the presence of other amino acids, starves the cell for S-adenosylmethionine and that it is the absence of S-adenosylmethionine and/or of C1-tetrahydrofolate derivatives that prevents normal cell division.

Links

PubMed Online version:10.1111/j.1365-2958.2008.06315.x

Keywords

Amino Acids; Cell Division; Culture Media; Escherichia coli; Escherichia coli Proteins; Gene Deletion; L-Serine Dehydratase; S-Adenosylmethionine

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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