Difference between revisions of "PMID:4882748"
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== Materials and Methods Used == | == Materials and Methods Used == | ||
| − | Parent Strain: | + | *Parent Strain: ML308 (''i<sup>-</sup>z<sup>+</sup>y<sup>+</sup>a<sup>+</sup>'') |
| + | *Membrane vesicle isolation | ||
| + | *TDG protection | ||
| + | *NEM labelling of LacY | ||
==Phenotype Annotations== | ==Phenotype Annotations== | ||
Revision as of 15:04, 10 December 2010
| Citation |
Carter, JR, Fox, CF and Kennedy, EP (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 60:725-32 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: ML308 (i-z+y+a+)
- Membrane vesicle isolation
- TDG protection
- NEM labelling of LacY
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
ML308-225 |
lacZ |
beta-galactosidase negative |
Metabolic Activity |
Biochemical Assay |
LacZ assay | |
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</protect>
