Difference between revisions of "PMID:4882748"
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==Main Points of the Paper == | ==Main Points of the Paper == | ||
| − | + | *The rate of the transport system in intact cells by NEM follows apparent first-order kinetics | |
| + | **M-TDG reaction: K<sub>D</sub>=5x10<sup>-5</sup> M | ||
| + | *The rate of the transport system in cell-free fractions by NEM was similar to that in intact cells | ||
| + | *Melibiose acts similarly to TDG in the NEM labelling, except that the affinity for the protein is lower | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
Revision as of 15:14, 10 December 2010
| Citation |
Carter, JR, Fox, CF and Kennedy, EP (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 60:725-32 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose |
| edit table |
Main Points of the Paper
- The rate of the transport system in intact cells by NEM follows apparent first-order kinetics
- M-TDG reaction: KD=5x10-5 M
- The rate of the transport system in cell-free fractions by NEM was similar to that in intact cells
- Melibiose acts similarly to TDG in the NEM labelling, except that the affinity for the protein is lower
Materials and Methods Used
- Parent Strain: ML308 (i-z+y+a+)
- Membrane vesicle isolation
- TDG protection
- NEM labelling of LacY
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
ML308-225 |
lacZ |
beta-galactosidase negative |
Metabolic Activity |
Biochemical Assay |
LacZ assay | |
| edit table |
</protect>
