Difference between revisions of "PMID:1320256"

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'''Morimyo, M, Hongo, E, Hama-Inaba, H and Machida, I'''  (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity.''Nucleic Acids Res.'' '''20''':3159-65
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!align=left  |Abstract
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A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (12.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1320256 PubMed]
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!align=left  |Keywords
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Amino Acid Sequence; Antiporters; Bacterial Proteins; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Membrane Proteins; Molecular Sequence Data; Paraquat; Plasmids; Restriction Mapping
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 14:31, 24 April 2012

Citation

Morimyo, M, Hongo, E, Hama-Inaba, H and Machida, I (1992) Cloning and characterization of the mvrC gene of Escherichia coli K-12 which confers resistance against methyl viologen toxicity.Nucleic Acids Res. 20:3159-65

Abstract

A new gene mvrC conferring resistance to methyl viologen, a powerful superoxide radical propagator, was cloned on 13.5 kilo base (kb) EcoRI DNA fragment. It gave resistance against methyl viologen to even a wild-type strain with gene dosage dependence. From the physical maps obtained by restriction enzyme digestions, it was predicted to locate at 580 kbp (12.3 min) on the physical map of E.coli. This was confirmed by the Southern hybridization of lambda phages covering this region with mvrC probe. The DNA sequence of mvrC gene was determined and its deduced protein encoding a 12 kd hydrophobic protein was confirmed by maxicell labeling of MvrC protein.

Links

PubMed

Keywords

Amino Acid Sequence; Antiporters; Bacterial Proteins; Base Sequence; Blotting, Southern; Cloning, Molecular; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Membrane Proteins; Molecular Sequence Data; Paraquat; Plasmids; Restriction Mapping

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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