Difference between revisions of "PMID:4577939"
(→Materials and Methods Used) |
(Table edited by Azweifel via TableEdit) |
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''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
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| − | + | inability to grow on lactose in a minimal salts medium | |
| | | | ||
| − | + | Growth | |
| | | | ||
energy uncoupled mutant | energy uncoupled mutant | ||
| | | | ||
| − | + | Other | |
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Revision as of 11:39, 13 December 2010
| Citation |
Kusch, M and Wilson, TH (1973) Defective lactose utilization by a mutant of Escherichia coli energy-uncoupled for lactose transport. The advantages of active transport versus facilitated diffusion.Biochim. Biophys. Acta 311:109-22 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport; Biological Transport, Active; Carbon Isotopes; Cell Division; Densitometry; Diffusion; Disaccharides; Enzyme Induction; Escherichia coli; Galactosidases; Kinetics; Lactose; Mutation; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-)
- Additional Strain: X9003 (i+z-y+a+, B1-)
- Additional Strain: X71-54 (i-z+yUNa-)- energy uncoupled mutant
- Additional Strain: 316 (i-z+y-a-)- y- strain
- β-galactosidase assay
- α-galactosidase assay
- Carrier-mediated transport assay
- thiogalactoside transacetylase activity was determined
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
inability to grow on lactose in a minimal salts medium |
Growth |
energy uncoupled mutant |
Other |
|
|
Escherichia coli |
NCBI:562 |
316 |
lacY- |
abolished transporter activity |
| |||
| edit table |
</protect>
