Difference between revisions of "PMID:4577939"
(Table edited by Azweifel via TableEdit) |
(Table edited by Azweifel via TableEdit) |
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| Line 73: | Line 73: | ||
''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
| | | | ||
| − | inability to grow on lactose | + | inability to grow on lactose |
| | | | ||
Growth | Growth | ||
| | | | ||
| − | + | in a minimal salts medium | |
| | | | ||
Other | Other | ||
| | | | ||
| − | + | in text, pg. 113 | |
|- | |- | ||
| | | | ||
| Line 92: | Line 92: | ||
''lacY<sup>-</sup>'' | ''lacY<sup>-</sup>'' | ||
| | | | ||
| − | + | no induction of alpha-galactosidase | |
| | | | ||
| − | + | Metabolic Activity | |
| | | | ||
| − | + | alpha-gal activity | |
| | | | ||
| − | + | Biochemical Assay | |
| | | | ||
| − | + | in text- pg. 116 | |
|- | |- | ||
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| Line 111: | Line 111: | ||
''lacY<sup>UN</sup>'' | ''lacY<sup>UN</sup>'' | ||
| | | | ||
| − | + | reduced rate of beta-galactosidase induction | |
| | | | ||
Metabolic Activity | Metabolic Activity | ||
| | | | ||
| − | beta-gal comparison b/t high and low levels of | + | beta-gal comparison b/t high and low levels of IPTG |
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| Line 140: | Line 140: | ||
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Figure 1- [<sup>14</sup>C]IPTG uptake | Figure 1- [<sup>14</sup>C]IPTG uptake | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | 54-41 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | reduced rate of beta-galactosidase induction | ||
| + | | | ||
| + | Metabolic Activity | ||
| + | | | ||
| + | |||
| + | | | ||
| + | Biochemical Assay | ||
| + | | | ||
| + | Figure 4- beta-galactosidase activity measured after lactose induction | ||
| + | |||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | X71-54 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | inability to grow on melibiose | ||
| + | | | ||
| + | Growth | ||
| + | | | ||
| + | in a minimal salts medium | ||
| + | | | ||
| + | Plating Assay | ||
| + | | | ||
| + | in text, pg. 115 | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:562 | ||
| + | | | ||
| + | X71-54 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | reduced rate of alpha-galactosidase induction | ||
| + | | | ||
| + | Metabolic Activity | ||
| + | | | ||
| + | |||
| + | | | ||
| + | Biochemical Assay | ||
| + | | | ||
| + | Figure 5- alpha-galactosidase activity measured after melibiose induction | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
Revision as of 12:27, 13 December 2010
| Citation |
Kusch, M and Wilson, TH (1973) Defective lactose utilization by a mutant of Escherichia coli energy-uncoupled for lactose transport. The advantages of active transport versus facilitated diffusion.Biochim. Biophys. Acta 311:109-22 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport; Biological Transport, Active; Carbon Isotopes; Cell Division; Densitometry; Diffusion; Disaccharides; Enzyme Induction; Escherichia coli; Galactosidases; Kinetics; Lactose; Mutation; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-)
- Additional Strain: X71-54 (i-z+yUNa-)- energy uncoupled mutant
- Additional Strain: X71-15 (i+z+y+a-)
- Additional Strain: X9003 (i+z-y+a+, B1-)
- Additional Strain: 54-41 (i+z+yUNa-)
- Additional Strain: 316 (i-z+y-a-)- y- strain
- β-galactosidase assay
- α-galactosidase assay
- Carrier-mediated transport assay
- thiogalactoside transacetylase activity was determined
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
inability to grow on lactose |
Growth |
in a minimal salts medium |
Other |
in text, pg. 113 |
|
Escherichia coli |
NCBI:562 |
316 |
lacY- |
no induction of alpha-galactosidase |
Metabolic Activity |
alpha-gal activity |
Biochemical Assay |
in text- pg. 116 |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
reduced rate of beta-galactosidase induction |
Metabolic Activity |
beta-gal comparison b/t high and low levels of IPTG |
Biochemical Assay |
Figure 2 and 3- Higher inducer concentrations were needed to achieve normal rates of induction |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
reduced intracellular IPTG accumulation |
Other |
mutant reached a maximum intracellular concentration of 0.1 mM compared to 0.4 mM IPTG for the energy-coupled parent |
Other |
Figure 1- [14C]IPTG uptake |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
reduced rate of beta-galactosidase induction |
Metabolic Activity |
Biochemical Assay |
Figure 4- beta-galactosidase activity measured after lactose induction | |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
inability to grow on melibiose |
Growth |
in a minimal salts medium |
Plating Assay |
in text, pg. 115 |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
reduced rate of alpha-galactosidase induction |
Metabolic Activity |
Biochemical Assay |
Figure 5- alpha-galactosidase activity measured after melibiose induction | |
| edit table |
</protect>
