Difference between revisions of "PMID:4577939"

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(Table edited by Azweifel via TableEdit)
(Table edited by Azweifel via TableEdit)
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''lacY<sup>UN</sup>''
 
''lacY<sup>UN</sup>''
 
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−
reduced rate of beta-galactosidase induction
+
White colonies on MacConkey melibiose indicator plates
 
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−
Metabolic Activity
+
Growth
 
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−
beta-gal comparison b/t high and low levels of IPTG
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inability to ferment 0.2% melibiose
  
 
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−
Biochemical Assay
+
Plating Assay
 
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−
Figure 2 and 3- Higher inducer concentrations were needed to achieve normal rates of induction
+
Table 4
 
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|-  
 
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''lacY<sup>UN</sup>''
 
''lacY<sup>UN</sup>''
 
|
 
|
−
inability to grow on melibiose
+
reduced growth rate on melibiose
 
|
 
|
 
Growth
 
Growth
Line 178: Line 178:
 
Plating Assay
 
Plating Assay
 
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−
in text, pg. 115
+
Table 3
 
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Figure 5- alpha-galactosidase activity measured after melibiose induction  
 
Figure 5- alpha-galactosidase activity measured after melibiose induction  
 +
|-
 +
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 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
54-41
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
decreased affinity for lactose
 +
|
 +
Other
 +
|
 +
 +
|
 +
Other
 +
|
 +
Table 5- kinetics of transporter for potential inducer molecules
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
54-41
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
Light pink colonies on MacConkey lactose indicator plates
 +
|
 +
Growth
 +
|
 +
reduced ability to ferment 0.2% lactose (fully restored when IPTG is present)
 +
 +
|
 +
Plating Assay
 +
|
 +
Table 4 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
X71-54
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
Light pink colonies on MacConkey melibiose indicator plates
 +
|
 +
Growth
 +
|
 +
inability to ferment 0.2% melibiose
 +
 +
|
 +
Plating Assay
 +
|
 +
Table 4 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
X71-54
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
Light pink colonies on MacConkey melibiose indicator plates
 +
|
 +
Growth
 +
|
 +
reduced ability to ferment 0.2% melibiose
 +
 +
|
 +
Plating Assay
 +
|
 +
Table 4 
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
54-41
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
no growth on lactose
 +
|
 +
Growth
 +
|
 +
in liquid culture with lactose concentrations b/t 5M and 50mM
 +
 +
|
 +
Plating Assay
 +
|
 +
in text, pg. 117- addition of 0.5mM IPTG added to lactose medium lead to growth comparable to parent with no constitutive cells
 +
|-
 +
|
 +
''Escherichia coli''
 +
|
 +
NCBI:562
 +
|
 +
54-41
 +
|
 +
''lacY<sup>UN</sup>''
 +
|
 +
increased affinity for melibiose
 +
|
 +
Other
 +
|
 +
 +
|
 +
Other
 +
|
 +
Table 5- kinetics of transporter for potential inducer molecules
  
 
|- class="tableEdit_footer"  
 
|- class="tableEdit_footer"  

Revision as of 12:55, 13 December 2010

Citation

Kusch, M and Wilson, TH (1973) Defective lactose utilization by a mutant of Escherichia coli energy-uncoupled for lactose transport. The advantages of active transport versus facilitated diffusion.Biochim. Biophys. Acta 311:109-22

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Biological Transport; Biological Transport, Active; Carbon Isotopes; Cell Division; Densitometry; Diffusion; Disaccharides; Enzyme Induction; Escherichia coli; Galactosidases; Kinetics; Lactose; Mutation; Transduction, Genetic

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

  • Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-)
  • Additional Strain: X71-54 (i-z+yUNa-)- energy uncoupled mutant
  • Additional Strain: X71-15 (i+z+y+a-)
  • Additional Strain: X9003 (i+z-y+a+, B1-)
  • Additional Strain: 54-41 (i+z+yUNa-)
  • Additional Strain: 316 (i-z+y-a-)- y- strain
  • β-galactosidase assay
  • α-galactosidase assay
  • Carrier-mediated transport assay
  • thiogalactoside transacetylase activity was determined
  • P1 Transduction

Phenotype Annotations

<protect>

Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

X71-54

lacYUN

inability to grow on lactose

Growth

in a minimal salts medium

Other

in text, pg. 113

Escherichia coli

NCBI:562

316

lacY-

no induction of alpha-galactosidase

Metabolic Activity

alpha-gal activity

Biochemical Assay

in text- pg. 116

Escherichia coli

NCBI:562

54-41

lacYUN

White colonies on MacConkey melibiose indicator plates

Growth

inability to ferment 0.2% melibiose

Plating Assay

Table 4

Escherichia coli

NCBI:562

54-41

lacYUN

reduced intracellular IPTG accumulation

Other

mutant reached a maximum intracellular concentration of 0.1 mM compared to 0.4 mM IPTG for the energy-coupled parent

Other

Figure 1- [14C]IPTG uptake

Escherichia coli

NCBI:562

54-41

lacYUN

reduced rate of beta-galactosidase induction

Metabolic Activity

Biochemical Assay

Figure 4- beta-galactosidase activity measured after lactose induction

Escherichia coli

NCBI:562

X71-54

lacYUN

reduced growth rate on melibiose

Growth

in a minimal salts medium

Plating Assay

Table 3

Escherichia coli

NCBI:562

X71-54

lacYUN

reduced rate of alpha-galactosidase induction

Metabolic Activity

Biochemical Assay

Figure 5- alpha-galactosidase activity measured after melibiose induction

Escherichia coli

NCBI:562

54-41

lacYUN

decreased affinity for lactose

Other

Other

Table 5- kinetics of transporter for potential inducer molecules

Escherichia coli

NCBI:562

54-41

lacYUN

Light pink colonies on MacConkey lactose indicator plates

Growth

reduced ability to ferment 0.2% lactose (fully restored when IPTG is present)

Plating Assay

Table 4

Escherichia coli

NCBI:562

X71-54

lacYUN

Light pink colonies on MacConkey melibiose indicator plates

Growth

inability to ferment 0.2% melibiose

Plating Assay

Table 4

Escherichia coli

NCBI:562

X71-54

lacYUN

Light pink colonies on MacConkey melibiose indicator plates

Growth

reduced ability to ferment 0.2% melibiose

Plating Assay

Table 4

Escherichia coli

NCBI:562

54-41

lacYUN

no growth on lactose

Growth

in liquid culture with lactose concentrations b/t 5M and 50mM

Plating Assay

in text, pg. 117- addition of 0.5mM IPTG added to lactose medium lead to growth comparable to parent with no constitutive cells

Escherichia coli

NCBI:562

54-41

lacYUN

increased affinity for melibiose

Other

Other

Table 5- kinetics of transporter for potential inducer molecules

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</protect>

Notes

Retrieved from "http://bcbp-ftq4xm2.biobio.tamu.edu/omp/dev/index.php?title=PMID:4577939&oldid=712"