Difference between revisions of "PMID:4933690"
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!|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes | !|Species!!Taxon ID!!Strain!!Gene (if known)!!OMP!!Phenotype!!Details!!Evidence!!Notes | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:83333 | ||
| + | | | ||
| + | X71-54 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | inability to accumulate thiomethylgalactoside | ||
| + | | | ||
| + | Other | ||
| + | | | ||
| + | Intracellular [<sup>14</sup>C]TMG accumulation | ||
| + | | | ||
| + | Other | ||
| + | | | ||
| + | Figure 1- Filter assay | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:83333 | ||
| + | | | ||
| + | X71-54 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | increased efflux rate of thiomethylgalactoside (TMG) | ||
| + | | | ||
| + | Other | ||
| + | | | ||
| + | exit rate of [<sup>14</sup>C]TMG was faster than that found for the parent | ||
| + | | | ||
| + | Other | ||
| + | | | ||
| + | Figure 2- Filter assay | ||
| + | |- | ||
| + | | | ||
| + | ''Escherichia coli'' | ||
| + | | | ||
| + | NCBI:83333 | ||
| + | | | ||
| + | X71-54 | ||
| + | | | ||
| + | ''lacY<sup>UN</sup>'' | ||
| + | | | ||
| + | increased rate of transporter substrate uptake | ||
| + | | | ||
| + | Metabolic Activity | ||
| + | | | ||
| + | 130% of normal uptake activity (gauged by ONPG entry) | ||
| + | | | ||
| + | Biochemical Assay | ||
| + | | | ||
| + | Table 1- ONPG assay | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
Revision as of 14:57, 13 December 2010
| Citation |
Wilson, TH, Kusch, M and Kashket, ER (1970) A mutant in Escherichia coli energy-uncoupled for lactose transport: a defect in the lactose-operon.Biochem. Biophys. Res. Commun. 40:1409-14 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport, Active; Carbon Isotopes; Cell Membrane; Coliphages; Escherichia coli; Galactose; Glycosides; Lactose; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-, F-)
- Additional Strain: X71-54
- Additional Strain: Ca77 (lactose-deleted, B1-, Hfr)
- β-galactosidase assay
- ONPG uptake assay
- Thiomethylgalactoside (TMG) transport assay
- P1 Transduction
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
inability to accumulate thiomethylgalactoside |
Other |
Intracellular [14C]TMG accumulation |
Other |
Figure 1- Filter assay |
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
increased efflux rate of thiomethylgalactoside (TMG) |
Other |
exit rate of [14C]TMG was faster than that found for the parent |
Other |
Figure 2- Filter assay |
|
Escherichia coli |
NCBI:83333 |
X71-54 |
lacYUN |
increased rate of transporter substrate uptake |
Metabolic Activity |
130% of normal uptake activity (gauged by ONPG entry) |
Biochemical Assay |
Table 1- ONPG assay |
| edit table |
</protect>
