Difference between revisions of "PMID:6355059"

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{|  id="U4fb69b50a8a0c"  class=" tableEdit PMID_info_table" 
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'''Stalmach, ME, Grothe, S and Wood, JM'''  (1983) Two proline porters in Escherichia coli K-12.''J. Bacteriol.'' '''156''':481-6
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!align=left  |Abstract
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Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6355059 PubMed]
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!align=left  |Keywords
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Amino Acid Transport Systems, Neutral; Escherichia coli; Genes, Bacterial; Genotype; Isoenzymes; Kinetics; Membrane Transport Proteins; Mutation; Proline; Species Specificity
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 13:56, 18 May 2012

Citation

Stalmach, ME, Grothe, S and Wood, JM (1983) Two proline porters in Escherichia coli K-12.J. Bacteriol. 156:481-6

Abstract

Escherichia coli mutants defective at putP and putA lack proline transport via proline porter I and proline dehydrogenase activity, respectively. They retain a proline uptake system (proline porter II) that is induced during tryptophan-limited growth and are sensitive to the toxic L-proline analog, 3,4-dehydroproline. 3,4-Dehydroproline-resistant mutants derived from a putP putA mutant lack proline porter II. Auxotrophic derivatives derived from putP+ or putP bacteria can grow if provided with proline at low concentration (25 microM); those derived from the 3,4-dehydroproline-resistant mutants require high proline for growth (2.5 mM). We conclude that E. coli, like Salmonella typhimurium, possesses a second proline porter that is inactivated by mutations at the proP locus.

Links

PubMed

Keywords

Amino Acid Transport Systems, Neutral; Escherichia coli; Genes, Bacterial; Genotype; Isoenzymes; Kinetics; Membrane Transport Proteins; Mutation; Proline; Species Specificity

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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