PMID:4892537

Citation	Kawasaki, T and Nose, Y (1969) Thiamine regulatory mutants in Escherichia coli.J. Biochem. 65:417-25
Abstract	No abstract in PubMed
Links	PubMed
Keywords	Adenine; Chromosome Mapping; Chromosomes, Bacterial; Culture Media; Escherichia coli; Mutation; Phosphotransferases; Pyrimidines; Thiamine; Thiazoles; Transferases
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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R1	Reference Condition:			0000005	enzyme assay data	Pyrithiamine resistant strains have lower activity of enzymes hydroxymethylprimidine kinase and phosphohydroxymethylpyrimidine kinase.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R3	Reference Condition:			0000005	enzyme assay data	Pyrithiamine resistant strains have lower activity of enzymes hydroxymethylprimidine kinase and phosphohydroxymethylpyrimidine kinase.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R1	Reference Condition:			0000005	enzyme assay data	Pyrithiamine resistant strains had 15 fold higher Thiaminephosphate pyrophosphorylase activity and 2 fold higher activity of Hydroxyethylthiazole kinase. See table II for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: k-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R3	Reference Condition:			0000005	enzyme assay data	Pyrithiamine resistant strains had 10 fold higher Thiaminephosphate pyrophosphorylase activity and 2 fold higher activity of Hydroxyethylthiazole kinase. See table II for full results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R1	Reference Condition:			0000005	enzyme assay data	Introduction of adenine to minimal medium causes increase activity of Hydroxymethylpyrimidine kinase, Phosphohydroxymethylpyrimidine kinase & Thiaminephosphate pyrophosphorylase within the Pyrithiamine resistant mutants. See Table IV for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thi + Genotype of Experimental Strain : thi mutation in PT-R3	Reference Condition:			0000005	enzyme assay data	Introduction of adenine to minimal medium causes increase activity of Hydroxymethylpyrimidine kinase, Phosphohydroxymethylpyrimidine kinase, Hydroxyethylthiazole kinase & Thiaminephosphate pyrophosphorylase within the Pyrithiamine resistant mutants. See Table IV for full experimental results.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thiC + Genotype of Experimental Strain : thiC mutation in PT-R1	Reference Condition:			0000005	enzyme assay data	Pyrithiamine resistance developed with a mutation to the thiC gene.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: AB1450 NCBI Taxon ID: 83333	Genotype of Reference Strain: thiC + Genotype of Experimental Strain : thiC mutation in PT-R1	Reference Condition:
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Notes

• thiamine regulatory mutants have defective control mechanisms for thiamine synthesis from hydroxymethylpyrimidine and hydroxyethylthiazole
• Strain PT-R1 required a concentration of Adenine 10 times higher than RT-r3 to depress thiamine synthesis (Table V)

References

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