Difference between revisions of "PMID:4994599"
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| + | {| id="M502a92928c2cf" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Burman, LG and Nordström, K''' (1971) Colicin tolerance induced by ampicillin or mutation to ampicillin resistance in a strain of Escherichia coli K-12.''J. Bacteriol.'' '''106''':1-13 | ||
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| + | !align=left |Abstract | ||
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| + | A mutant (G11el) of Escherichia coli selected as being resistant to ampicillin and showing signs of an envelope defect was also found to be tolerant to colicins E2 and E3. The colicin tolerance of G11el could be partially repressed by Mg(2+) ions. Transition from tolerance to sensitivity and vice versa by shifting the concentration of Mg(2+) in the growth medium required several generations. This indicated that synthesis of new envelope material was needed for transition. Previous physiological results have indicated a change in the envelope lipopolysaccharide (LPS) of G11el. However, chemical analyses revealed no differences in carbohydrate composition between LPS from G11el and its parent strain G11al. Genetic experiments showed that the mutation in G11el is located at about 20 min on the E. coli K-12 chromosome. The mutation was dominant over wild type in partial diploids with the mutation located on the episome. Because colicin tolerance was the most striking phenotypic effect as a result of mutation in the actual locus, this gene will be named tolD until the exact gene product is known. Spheroplasts formed from G11al and G11el by ethylenediaminetetraacetate-lysozyme treatment did not adsorb colicin E2; however, penicillin spheroplasts of G11al and G11el were tolerant to colicin E2. Thus, colicin tolerance can be induced biochemically. It is suggested that colicin tolerance often is a secondary consequence of a change in the cell envelope. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4994599 PubMed] | ||
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| + | |- | ||
| + | !align=left |Keywords | ||
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| + | Ampicillin; Cell Wall; Chromatography, Gas; Chromosome Mapping; Chromosomes, Bacterial; Colicins; Crosses, Genetic; Culture Media; Edetic Acid; Escherichia coli; Genes; Genetics, Microbial; Hexosamines; Lipopolysaccharides; Magnesium; Monosaccharides; Muramidase; Mutation; Penicillin Resistance; Phenotype; Phosphates; Polysaccharides, Bacterial; Protoplasts; Recombination, Genetic; Trypsin | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3268.M502a92928c2cf--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
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| + | {| id="R502a9292b49c3" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3268.R502a9292b49c3--></protect> | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | |||
| + | [[Category:Publication]] | ||
Revision as of 13:01, 14 August 2012
| Citation |
Burman, LG and Nordström, K (1971) Colicin tolerance induced by ampicillin or mutation to ampicillin resistance in a strain of Escherichia coli K-12.J. Bacteriol. 106:1-13 |
|---|---|
| Abstract |
A mutant (G11el) of Escherichia coli selected as being resistant to ampicillin and showing signs of an envelope defect was also found to be tolerant to colicins E2 and E3. The colicin tolerance of G11el could be partially repressed by Mg(2+) ions. Transition from tolerance to sensitivity and vice versa by shifting the concentration of Mg(2+) in the growth medium required several generations. This indicated that synthesis of new envelope material was needed for transition. Previous physiological results have indicated a change in the envelope lipopolysaccharide (LPS) of G11el. However, chemical analyses revealed no differences in carbohydrate composition between LPS from G11el and its parent strain G11al. Genetic experiments showed that the mutation in G11el is located at about 20 min on the E. coli K-12 chromosome. The mutation was dominant over wild type in partial diploids with the mutation located on the episome. Because colicin tolerance was the most striking phenotypic effect as a result of mutation in the actual locus, this gene will be named tolD until the exact gene product is known. Spheroplasts formed from G11al and G11el by ethylenediaminetetraacetate-lysozyme treatment did not adsorb colicin E2; however, penicillin spheroplasts of G11al and G11el were tolerant to colicin E2. Thus, colicin tolerance can be induced biochemically. It is suggested that colicin tolerance often is a secondary consequence of a change in the cell envelope. |
| Links | |
| Keywords |
Ampicillin; Cell Wall; Chromatography, Gas; Chromosome Mapping; Chromosomes, Bacterial; Colicins; Crosses, Genetic; Culture Media; Edetic Acid; Escherichia coli; Genes; Genetics, Microbial; Hexosamines; Lipopolysaccharides; Magnesium; Monosaccharides; Muramidase; Mutation; Penicillin Resistance; Phenotype; Phosphates; Polysaccharides, Bacterial; Protoplasts; Recombination, Genetic; Trypsin |
| edit table |
Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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