Difference between revisions of "PMID:390093"

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{|  id="F504cae3f9b997"  class=" tableEdit PMID_info_table" 
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'''Săsărman, A, Chartrand, P, Lavoie, M, Tardif, D, Proschek, R and Lapointe, C'''  (1979) Mapping of a new hem gene in Escherichia coli K12.''J. Gen. Microbiol.'' '''113''':297-303
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!align=left  |Abstract
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A new type of haem-deficient mutant was isolated in Escherichia coli K12 by neomycin selection. The mutant, designated SASX38, accumulated uroporphyrin, coproporphyrin and protoporphyrin. Since it possessed normal ferrochelatase activity, it was assumed to be deficient in protoporphyrinogen oxidase activity. The gene affected in the mutant was designated hemG. Mapping of the hemG gene by phage P1-mediated transduction showed that it was located very close to the chlB gene (frequency of cotransduction 78.7%), between the metE and rha markers. This location is distinct from the other known hem loci in E. coli K12.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=390093 PubMed]
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!align=left  |Keywords
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Chromosome Mapping; Escherichia coli; Escherichia coli Proteins; Ferrochelatase; Heme; Mutation; Oxidoreductases; Porphyrins; Protoporphyrinogen Oxidase; Protoporphyrins; Transduction, Genetic
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==Main Points of the Paper ==
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{{LitSignificance}}
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== Materials and Methods Used ==
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{{LitMaterials}}
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 09:57, 9 September 2012

Citation

Săsărman, A, Chartrand, P, Lavoie, M, Tardif, D, Proschek, R and Lapointe, C (1979) Mapping of a new hem gene in Escherichia coli K12.J. Gen. Microbiol. 113:297-303

Abstract

A new type of haem-deficient mutant was isolated in Escherichia coli K12 by neomycin selection. The mutant, designated SASX38, accumulated uroporphyrin, coproporphyrin and protoporphyrin. Since it possessed normal ferrochelatase activity, it was assumed to be deficient in protoporphyrinogen oxidase activity. The gene affected in the mutant was designated hemG. Mapping of the hemG gene by phage P1-mediated transduction showed that it was located very close to the chlB gene (frequency of cotransduction 78.7%), between the metE and rha markers. This location is distinct from the other known hem loci in E. coli K12.

Links

PubMed

Keywords

Chromosome Mapping; Escherichia coli; Escherichia coli Proteins; Ferrochelatase; Heme; Mutation; Oxidoreductases; Porphyrins; Protoporphyrinogen Oxidase; Protoporphyrins; Transduction, Genetic

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

See Help:AnnotationTable for details on how to edit this table.
<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status

</protect>

Notes

References

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