Difference between revisions of "PMID:5119203"

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'''Goldschmidt, MC  and Lockhart, BM '''  (1971) Rapid methods for determining decarboxylase activity: arginine decarboxylase. ''Appl Microbiol'' '''22''':350-7
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!align=left  |Abstract
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A rapid biochemical method for the determination of arginine decarboxylase (EC 4.1.1.19) activity has been developed for use in the routine clinical microbiology laboratory and correlated with similar procedures for ornithine and lysine decarboxylase (EC 4.1.1.18) systems. It is based on the detection of agmatine, the amine end product formed during growth on a synthetic medium containing arginine as the key amino acid. A modified diacetyl reagent is used to detect this amine after a differential butanol extraction of the cultures. This procedure can be used to detect this amine after a 1- to 4-hr incubation period (with the use of an initial concentrated inoculum) or with an overnight culture. Thus, both an indirect measurement based on the alkalinization of the medium and a lengthy incubation period were avoided. Parameters for optimal enzyme activity and the pertinent enzyme systems involved in arginine and agmatine catabolism are discussed in detail.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=5119203 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC376313 PMC376313]
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Alcohols; Amines/analysis; Amines/biosynthesis; Arginase/metabolism; Arginine/metabolism; Bacteria/classification; Bacteria/enzymology; Bacteria/metabolism; Carboxy-Lyases/metabolism; Chromatography, Thin Layer; Culture Media; Decarboxylation; Enterobacteriaceae/classification; Enterobacteriaceae/enzymology; Enterobacteriaceae/metabolism; Evaluation Studies as Topic; Guanidines/analysis; Guanidines/biosynthesis; Hydrogen-Ion Concentration; Hydrolases/metabolism; Indicators and Reagents; Methods; Oxygen; Spectrophotometry; Temperature
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 17:19, 14 September 2012

Citation

Goldschmidt, MC and Lockhart, BM (1971) Rapid methods for determining decarboxylase activity: arginine decarboxylase. Appl Microbiol 22:350-7

Abstract

A rapid biochemical method for the determination of arginine decarboxylase (EC 4.1.1.19) activity has been developed for use in the routine clinical microbiology laboratory and correlated with similar procedures for ornithine and lysine decarboxylase (EC 4.1.1.18) systems. It is based on the detection of agmatine, the amine end product formed during growth on a synthetic medium containing arginine as the key amino acid. A modified diacetyl reagent is used to detect this amine after a differential butanol extraction of the cultures. This procedure can be used to detect this amine after a 1- to 4-hr incubation period (with the use of an initial concentrated inoculum) or with an overnight culture. Thus, both an indirect measurement based on the alkalinization of the medium and a lengthy incubation period were avoided. Parameters for optimal enzyme activity and the pertinent enzyme systems involved in arginine and agmatine catabolism are discussed in detail.

Links

PubMed PMC376313

Keywords

Alcohols; Amines/analysis; Amines/biosynthesis; Arginase/metabolism; Arginine/metabolism; Bacteria/classification; Bacteria/enzymology; Bacteria/metabolism; Carboxy-Lyases/metabolism; Chromatography, Thin Layer; Culture Media; Decarboxylation; Enterobacteriaceae/classification; Enterobacteriaceae/enzymology; Enterobacteriaceae/metabolism; Evaluation Studies as Topic; Guanidines/analysis; Guanidines/biosynthesis; Hydrogen-Ion Concentration; Hydrolases/metabolism; Indicators and Reagents; Methods; Oxygen; Spectrophotometry; Temperature

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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