Difference between revisions of "PMID:6378096"
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| + | {| id="B5054eb8a2a239" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Feary, TW and Mayo, JA ''' (1984) Detection of streptococcal mutants presumed to be defective in sugar catabolism. ''Appl. Environ. Microbiol.'' '''47''':1348-51 | ||
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| + | !align=left |Abstract | ||
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| + | The tetrazolium method for detection of bacterial mutants defective in sugar catabolism was modified for use with streptococci. The critical factors were (i) the concentration of tetrazolium, which must be titrated to determine the optimum concentration for each species or even strain, and (ii) anaerobic incubation of tetrazolium-containing agar plates. When used with standard mutagenesis protocols, this method yielded lactose-negative mutants of nine streptococcal strains representing six species. A collection of lactose-negative mutants of streptococcus, sanguis Challis was characterized and contained phospho-beta-galactosidase, lactose phosphotransferase, and general phosphotransferase mutants. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6378096 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC240244 PMC240244] | ||
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| + | !align=left |Keywords | ||
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| + | Bacteriological Techniques; Culture Media; Glucose/metabolism; Lac Operon; Lactose/metabolism; Mutation; Phenotype; Streptococcus sanguis/genetics; Streptococcus sanguis/metabolism | ||
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| + | <!--box uid=d41d8cd98f00b204e9800998ecf8427e.3354.B5054eb8a2a239--> | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Latest revision as of 15:56, 15 September 2012
| Citation |
Feary, TW and Mayo, JA (1984) Detection of streptococcal mutants presumed to be defective in sugar catabolism. Appl. Environ. Microbiol. 47:1348-51 |
|---|---|
| Abstract |
The tetrazolium method for detection of bacterial mutants defective in sugar catabolism was modified for use with streptococci. The critical factors were (i) the concentration of tetrazolium, which must be titrated to determine the optimum concentration for each species or even strain, and (ii) anaerobic incubation of tetrazolium-containing agar plates. When used with standard mutagenesis protocols, this method yielded lactose-negative mutants of nine streptococcal strains representing six species. A collection of lactose-negative mutants of streptococcus, sanguis Challis was characterized and contained phospho-beta-galactosidase, lactose phosphotransferase, and general phosphotransferase mutants. |
| Links | |
| Keywords |
Bacteriological Techniques; Culture Media; Glucose/metabolism; Lac Operon; Lactose/metabolism; Mutation; Phenotype; Streptococcus sanguis/genetics; Streptococcus sanguis/metabolism |
| edit table |
Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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