Difference between revisions of "PMID:7538512"

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'''Johnson, MJ , Thatcher, E  and Cox, ME '''  (1995) Techniques for controlling variability in gram staining of obligate anaerobes. ''J. Clin. Microbiol.'' '''33''':755-8
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!align=left  |Abstract
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Identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; Peptostreptococcus asaccharolyticus, Eubacterium plautii, Clostridium ramosum, Clostridium symbiosum, and Clostridium clostridiiforme are among the nonconformists with regard to conventional Gram-staining procedures. Accurate Gram staining of American Type Culture Collection strains of these anaerobic bacteria is possible by implementing fixing and staining techniques within a gloveless anaerobic chamber. Under anaerobic conditions, gram-positive staining occurred in all test organisms with "quick" fixing techniques with both absolute methanol and formalin. The results support the hypothesis that, when anaerobic bacteria are exposed to oxygen, a breakdown of the physical integrity of the cell wall occurs, introducing Gram stain variability in gram-positive anaerobes.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7538512 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC228030 PMC228030]
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Bacteria, Anaerobic/isolation & purification; Fixatives; Formaldehyde; Gentian Violet/diagnostic use; Gram-Positive Bacteria/isolation & purification; Methanol; Phenazines/diagnostic use; Reproducibility of Results; Sensitivity and Specificity; Staining and Labeling/methods
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 16:07, 15 September 2012

Citation

Johnson, MJ , Thatcher, E and Cox, ME (1995) Techniques for controlling variability in gram staining of obligate anaerobes. J. Clin. Microbiol. 33:755-8

Abstract

Identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; Peptostreptococcus asaccharolyticus, Eubacterium plautii, Clostridium ramosum, Clostridium symbiosum, and Clostridium clostridiiforme are among the nonconformists with regard to conventional Gram-staining procedures. Accurate Gram staining of American Type Culture Collection strains of these anaerobic bacteria is possible by implementing fixing and staining techniques within a gloveless anaerobic chamber. Under anaerobic conditions, gram-positive staining occurred in all test organisms with "quick" fixing techniques with both absolute methanol and formalin. The results support the hypothesis that, when anaerobic bacteria are exposed to oxygen, a breakdown of the physical integrity of the cell wall occurs, introducing Gram stain variability in gram-positive anaerobes.

Links

PubMed PMC228030

Keywords

Bacteria, Anaerobic/isolation & purification; Fixatives; Formaldehyde; Gentian Violet/diagnostic use; Gram-Positive Bacteria/isolation & purification; Methanol; Phenazines/diagnostic use; Reproducibility of Results; Sensitivity and Specificity; Staining and Labeling/methods

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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