Difference between revisions of "PMID:7694852"

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{|  id="U5054fe365a09d"  class=" tableEdit PMID_info_table" 
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'''Drancourt, M  and Raoult, D '''  (1993) Proposed tests for the routine identification of Rochalimaea species. ''Eur. J. Clin. Microbiol. Infect. Dis.'' '''12''':710-3
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!align=left  |Abstract
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A study was conducted to establish tests for the routine identification of Rochalimaea species. Strains used were reference strains of Rochalimaea vinsonii and Rochalimaea quintana, and a type strain and six human isolates of Rochalimaea henselae. Rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on MacConkey agar. Further testing of the organisms in a commercial identification system with the addition of hemin (100 micrograms/ml) to the medium revealed biochemical reactivity of the organisms not previously observed. The Voges-Proskauer reaction, tests for hydrolysis of hippurate and esculin, leucine arylamidase activity and the lactose test allowed identification and differentiation of the three species. Rochalimaea henselae was the only species with a positive lactose test and Rochalimaea quintana was the only species with a positive Voges-Proskauer reaction. Further studies are needed to confirm the validity of these tests for identification of Rochalimaea species.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7694852 PubMed]
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!align=left  |Keywords
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Bacterial Typing Techniques; Carbohydrate Metabolism; Culture Media; Gentian Violet; Humans; Phenazines; Rickettsiaceae/classification; Rickettsiaceae/metabolism; Rickettsiaceae/physiology; Species Specificity; Staining and Labeling
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 17:16, 15 September 2012

Citation

Drancourt, M and Raoult, D (1993) Proposed tests for the routine identification of Rochalimaea species. Eur. J. Clin. Microbiol. Infect. Dis. 12:710-3

Abstract

A study was conducted to establish tests for the routine identification of Rochalimaea species. Strains used were reference strains of Rochalimaea vinsonii and Rochalimaea quintana, and a type strain and six human isolates of Rochalimaea henselae. Rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on MacConkey agar. Further testing of the organisms in a commercial identification system with the addition of hemin (100 micrograms/ml) to the medium revealed biochemical reactivity of the organisms not previously observed. The Voges-Proskauer reaction, tests for hydrolysis of hippurate and esculin, leucine arylamidase activity and the lactose test allowed identification and differentiation of the three species. Rochalimaea henselae was the only species with a positive lactose test and Rochalimaea quintana was the only species with a positive Voges-Proskauer reaction. Further studies are needed to confirm the validity of these tests for identification of Rochalimaea species.

Links

PubMed

Keywords

Bacterial Typing Techniques; Carbohydrate Metabolism; Culture Media; Gentian Violet; Humans; Phenazines; Rickettsiaceae/classification; Rickettsiaceae/metabolism; Rickettsiaceae/physiology; Species Specificity; Staining and Labeling

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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