Difference between revisions of "PMID:4887874"
(→Main Points of the Paper) |
(→Materials and Methods Used) |
||
| Line 42: | Line 42: | ||
== Materials and Methods Used == | == Materials and Methods Used == | ||
*Parent Strain: NG545 (''trp-8'', Sm<sup>S</sup> Hfr) | *Parent Strain: NG545 (''trp-8'', Sm<sup>S</sup> Hfr) | ||
| + | **UAG mutation had not been altered in the suppressor strains isolated | ||
*Strain: CA77B (''Δlac, trp-8'', Sm<sup>R</sup> F<sup>-</sup>) | *Strain: CA77B (''Δlac, trp-8'', Sm<sup>R</sup> F<sup>-</sup>) | ||
*Strain: X103B (''Δlac pro, trp-8'', Sm<sup>R</sup> F<sup>-</sup>) | *Strain: X103B (''Δlac pro, trp-8'', Sm<sup>R</sup> F<sup>-</sup>) | ||
Revision as of 17:18, 15 December 2010
| Citation |
Grodzicker, T and Zipser, D (1968) A mutation which creates a new site for the re-initiation of polypeptide synthesis in the z gene of the lac operon of Escherichia coli.J. Mol. Biol. 38:305-14 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Acyltransferases; Chromosome Mapping; Escherichia coli; Genes; Membrane Transport Proteins; Mutation; Operon |
| edit table |
Main Points of the Paper
- Polar effects of a UAG mutation in the operator-proximal region of the z gene are studied which leads to the isolation of two suppressor mutations
- π, a new site of initiation of protein synthesis is described
- operates efficiently to restore permease and acetylase activity to a high level when induced
- regulation/induction is still required, so the authors deduce that π is a new site of translation initiation (
- not a new site of transcriptional initiation because acetylase is not present in the absence of inducer
Materials and Methods Used
- Parent Strain: NG545 (trp-8, SmS Hfr)
- UAG mutation had not been altered in the suppressor strains isolated
- Strain: CA77B (Δlac, trp-8, SmR F-)
- Strain: X103B (Δlac pro, trp-8, SmR F-)
- Strain: M107 (Δlac, suI+, SmR F-)
- Strain: ΔLacW (Δlac, his-, SmR F-)
- Strain: E29 (lac+ , F'/lac+, threonine-, leucine-, B1-, SmR)
- Acetylase assay
- β-galactosidase assay
Phenotype Annotations
See Help:AnnotationTable for details on how to edit this table.
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)a |
lacZ |
able to metabolize melibiose |
Growth |
growth on melibiose- polar effects |
Plating Assay |
Table 2 |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1) |
lacZ |
Growth |
able to metabolize lactose only in a suI+ background |
Plating Assay |
Table 2- UAG polar mutation | |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)b |
lacZ |
unable to metabolize melibiose |
Growth |
growth on melibiose-polar effects |
Plating Assay |
Table 2- |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)a |
lacZ |
reduced beta-galactosidase activity |
Metabolic Activity |
trace amounts when induced |
Biochemical Assay |
Table 2 |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)b |
lacZ |
reduced beta-galactosidase activity |
Metabolic Activity |
trace amounts when induced |
Biochemical Assay |
Table 2 |
|
Escherichia coli |
NCBI:562 |
NG545B |
lacZ |
reduced acetylase activity |
Metabolic Activity |
4.2% of the wild type level when induced |
Biochemical Assay |
Tables 3 and 4 |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)aB |
lacZ |
reduced acetylase activity |
Metabolic Activity |
35% of the wild type level when induced |
Biochemical Assay |
Tables 3 and 4 |
|
Escherichia coli |
NCBI:562 |
NG545-pi(1)bB |
lacZ |
reduced acetylase activity |
Metabolic Activity |
33.5% of the wild type level when induced |
Biochemical Assay |
Tables 3 and 4 |
| edit table |
</protect>
Notes
References
See Help:References for how to manage references in omp dev.
