Difference between revisions of "PMID:4607764"
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| + | {| id="R50564e50a04e7" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Silhavy, TJ and Boos, W ''' (1974) Selection procedure for mutants defective in the beta-methylgalactoside transport system of Escherichia coli utilizing the compound 2R-glyceryl-beta-D-galactopyranoside. ''J. Bacteriol.'' '''120''':424-32 | ||
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| + | !align=left |Abstract | ||
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| + | A procedure has been devised that allows selection of mutants defective in the beta-methylgalactoside transport system (mgl) of Escherichia coli. This procedure utilizes the compound 2R-glyceryl-beta-d-galactopyranoside (glycerylgalactoside), which is known to be transported by only two transport system in E. coli, namely, the lactose and the beta-methylgalactoside transport systems. Mutants lacking glycerol-3-phosphate dehydrogenase (glpD) are sensitive to glycerol. Similarly, mutants lacking uridine diphosphate-galactose-4-epimerase (galE) are sensitive to galactose. Glycerylgalactoside is an inducer of the lactose operon and also a substrate for beta-galactosidase. Thus, a mgl(+)glpD galE lacY strain will not grow in the presence of glycerylgalactoside owing to accumulated glycerol-3-phosphate, galactose-1-phosphate, and uridine diphosphate-galactose. We have constructed such a strain and shown that mgl mutants can be obtained by selecting for those that grow in the presence of glycerylgalactoside. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4607764 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC245779 PMC245779] | ||
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| + | !align=left |Keywords | ||
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| + | Autoradiography; Biological Transport, Active; Carbohydrate Epimerases/biosynthesis; Carbon Radioisotopes; Chromatography, Paper; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactose/metabolism; Galactosidases/biosynthesis; Glycerol/metabolism; Glycerolphosphate Dehydrogenase/biosynthesis; Glycosides/metabolism; Kinetics; Methylgalactosides/metabolism; Methylglycosides/metabolism; Mutagens; Mutation; Nitrosoguanidines; Succinates/metabolism; Transduction, Genetic | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
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| + | {| id="H50564e50ca62f" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
| + | |||
| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | |||
| + | [[Category:Publication]] | ||
Latest revision as of 17:10, 16 September 2012
| Citation |
Silhavy, TJ and Boos, W (1974) Selection procedure for mutants defective in the beta-methylgalactoside transport system of Escherichia coli utilizing the compound 2R-glyceryl-beta-D-galactopyranoside. J. Bacteriol. 120:424-32 |
|---|---|
| Abstract |
A procedure has been devised that allows selection of mutants defective in the beta-methylgalactoside transport system (mgl) of Escherichia coli. This procedure utilizes the compound 2R-glyceryl-beta-d-galactopyranoside (glycerylgalactoside), which is known to be transported by only two transport system in E. coli, namely, the lactose and the beta-methylgalactoside transport systems. Mutants lacking glycerol-3-phosphate dehydrogenase (glpD) are sensitive to glycerol. Similarly, mutants lacking uridine diphosphate-galactose-4-epimerase (galE) are sensitive to galactose. Glycerylgalactoside is an inducer of the lactose operon and also a substrate for beta-galactosidase. Thus, a mgl(+)glpD galE lacY strain will not grow in the presence of glycerylgalactoside owing to accumulated glycerol-3-phosphate, galactose-1-phosphate, and uridine diphosphate-galactose. We have constructed such a strain and shown that mgl mutants can be obtained by selecting for those that grow in the presence of glycerylgalactoside. |
| Links | |
| Keywords |
Autoradiography; Biological Transport, Active; Carbohydrate Epimerases/biosynthesis; Carbon Radioisotopes; Chromatography, Paper; Escherichia coli/enzymology; Escherichia coli/isolation & purification; Escherichia coli/metabolism; Galactose/metabolism; Galactosidases/biosynthesis; Glycerol/metabolism; Glycerolphosphate Dehydrogenase/biosynthesis; Glycosides/metabolism; Kinetics; Methylgalactosides/metabolism; Methylglycosides/metabolism; Mutagens; Mutation; Nitrosoguanidines; Succinates/metabolism; Transduction, Genetic |
| edit table |
Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
References
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