Difference between revisions of "PMID:2480362"

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'''Gaston, MA  and Pitt, TL '''  (1989) Improved O-serotyping method for Serratia marcescens. ''J. Clin. Microbiol.'' '''27''':2702-5
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In a previous study, we found that some O serotypes of Serratia marcescens, as defined by agglutination tests, were not based on lipopolysaccharide (LPS) O antigens. We developed a dot enzyme immunoassay with a high degree of LPS specificity and tested 104 distinct clinical strains. Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21 (12.5%), O8 (8.7%), O6/O7 (5.8%), O4 (3.8%), O18 (2.9%), and O9 (2.9%). Two new antigens, S1254 (13.5%) and S3255 (3.8%), were also found. Agglutination tests with O antisera identified the LPS antigen in only 36 strains. Prodigiosin production was restricted to serotypes O8, O6, and S3255 and strains with a rough or semirough LPS phenotype. Dot immunoassay appears to offer greater accuracy than agglutination tests for serotype identification in S. marcescens.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2480362 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC267112 PMC267112]
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Agglutination Tests; Antigens, Bacterial; False Positive Reactions; Humans; Immune Sera/immunology; Immunoblotting; Immunoenzyme Techniques; Lipopolysaccharides/immunology; O Antigens; Phenotype; Predictive Value of Tests; Serotyping; Serratia marcescens/classification; Serratia marcescens/immunology
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 18:56, 16 September 2012

Citation

Gaston, MA and Pitt, TL (1989) Improved O-serotyping method for Serratia marcescens. J. Clin. Microbiol. 27:2702-5

Abstract

In a previous study, we found that some O serotypes of Serratia marcescens, as defined by agglutination tests, were not based on lipopolysaccharide (LPS) O antigens. We developed a dot enzyme immunoassay with a high degree of LPS specificity and tested 104 distinct clinical strains. Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21 (12.5%), O8 (8.7%), O6/O7 (5.8%), O4 (3.8%), O18 (2.9%), and O9 (2.9%). Two new antigens, S1254 (13.5%) and S3255 (3.8%), were also found. Agglutination tests with O antisera identified the LPS antigen in only 36 strains. Prodigiosin production was restricted to serotypes O8, O6, and S3255 and strains with a rough or semirough LPS phenotype. Dot immunoassay appears to offer greater accuracy than agglutination tests for serotype identification in S. marcescens.

Links

PubMed PMC267112

Keywords

Agglutination Tests; Antigens, Bacterial; False Positive Reactions; Humans; Immune Sera/immunology; Immunoblotting; Immunoenzyme Techniques; Lipopolysaccharides/immunology; O Antigens; Phenotype; Predictive Value of Tests; Serotyping; Serratia marcescens/classification; Serratia marcescens/immunology

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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