Difference between revisions of "PMID:19943910"

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'''Christensen-Dalsgaard, M , Jørgensen, MG  and Gerdes, K '''  (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. ''Mol. Microbiol.'' '''75''':333-48
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Prokaryotic toxin - antitoxin (TA) loci encode mRNA interferases that inhibit translation, either by cleaving mRNA codons at the ribosomal A site or by cleaving any RNA site-specifically. So far, seven mRNA interferases of Escherichia coli have been identified, four of which cleave mRNA by a translation-dependent mechanism. Here, we experimentally confirmed the presence of three novel TA loci in E. coli. We found that the yafNO, higBA (ygjNM) and ygiUT loci encode mRNA interferases related to RelE. YafO and HigB cleaved translated mRNA only, while YgiU cleaved RNA site-specifically at GC[A/U], independently of translation. Thus, YgiU is the first RelE-related mRNA interferase that cleaves mRNA independently of translation, in vivo. All three loci were induced by amino acid starvation, and inhibition of translation although to different degrees. Carbon starvation induced only two of the loci. The yafNO locus was induced by DNA damage, but the transcription originated from the dinB promoter. Thus, our results showed that the different TA loci responded differentially to environmental stresses. Induction of the three loci depended on Lon protease that may sense the environmental stresses and activate TA loci by cleavage of the antitoxins. Transcription of the three TA operons was autoregulated by the antitoxins.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=19943910 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2814082 PMC2814082]
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Online version:[http://dx.doi.org/10.1111/j.1365-2958.2009.06969.x 10.1111/j.1365-2958.2009.06969.x]
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Antitoxins/genetics; Antitoxins/pharmacology; Bacterial Toxins/chemistry; Bacterial Toxins/genetics; Bacterial Toxins/toxicity; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/genetics; Colony-Forming Units Assay; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Environment; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Messenger/genetics; Sequence Homology
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 10:51, 17 September 2012

Citation

Christensen-Dalsgaard, M , Jørgensen, MG and Gerdes, K (2010) Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses. Mol. Microbiol. 75:333-48

Abstract

Prokaryotic toxin - antitoxin (TA) loci encode mRNA interferases that inhibit translation, either by cleaving mRNA codons at the ribosomal A site or by cleaving any RNA site-specifically. So far, seven mRNA interferases of Escherichia coli have been identified, four of which cleave mRNA by a translation-dependent mechanism. Here, we experimentally confirmed the presence of three novel TA loci in E. coli. We found that the yafNO, higBA (ygjNM) and ygiUT loci encode mRNA interferases related to RelE. YafO and HigB cleaved translated mRNA only, while YgiU cleaved RNA site-specifically at GC[A/U], independently of translation. Thus, YgiU is the first RelE-related mRNA interferase that cleaves mRNA independently of translation, in vivo. All three loci were induced by amino acid starvation, and inhibition of translation although to different degrees. Carbon starvation induced only two of the loci. The yafNO locus was induced by DNA damage, but the transcription originated from the dinB promoter. Thus, our results showed that the different TA loci responded differentially to environmental stresses. Induction of the three loci depended on Lon protease that may sense the environmental stresses and activate TA loci by cleavage of the antitoxins. Transcription of the three TA operons was autoregulated by the antitoxins.

Links

PubMed PMC2814082 Online version:10.1111/j.1365-2958.2009.06969.x

Keywords

Antitoxins/genetics; Antitoxins/pharmacology; Bacterial Toxins/chemistry; Bacterial Toxins/genetics; Bacterial Toxins/toxicity; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial/genetics; Colony-Forming Units Assay; DNA, Bacterial/chemistry; DNA, Bacterial/genetics; Environment; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; RNA, Bacterial/genetics; RNA, Messenger/genetics; Sequence Homology

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Main Points of the Paper

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Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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