Difference between revisions of "PMID:1102522"

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'''Robertson, J , Gomersall, M  and Gill, P '''  (1975) Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. ''J. Bacteriol.'' '''124''':1007-18
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!align=left  |Abstract
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Improved methods for studying the growth of Mycoplasma hominis (ATCC 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. Cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. Similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefore seems characteristic of the species. The pleomorphic populations contain small forms having diameters within the 100- to 250-nm size range reported for "elementary bodies." Such forms were isolated from this strain of M. hominis by sequential filtration using gravity alone, after cell aggregates were dispersed by Pronase treatment. Of the small bodies which traversed membranes of 220-nm pore size, a negligible number grew in liquid or on solid media, suggesting that these were not essential reproductive units in a life cycle, but involution forms due to growth in an altered environment.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1102522 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC235991 PMC235991]
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Bacteriological Techniques; Cell Division; Deoxyribonucleases; Mycoplasma/growth & development; Mycoplasma/isolation & purification; Mycoplasma/ultrastructure; Pronase; Sonication; Trypsin
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Latest revision as of 11:26, 17 September 2012

Citation

Robertson, J , Gomersall, M and Gill, P (1975) Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. J. Bacteriol. 124:1007-18

Abstract

Improved methods for studying the growth of Mycoplasma hominis (ATCC 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. Cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. Similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefore seems characteristic of the species. The pleomorphic populations contain small forms having diameters within the 100- to 250-nm size range reported for "elementary bodies." Such forms were isolated from this strain of M. hominis by sequential filtration using gravity alone, after cell aggregates were dispersed by Pronase treatment. Of the small bodies which traversed membranes of 220-nm pore size, a negligible number grew in liquid or on solid media, suggesting that these were not essential reproductive units in a life cycle, but involution forms due to growth in an altered environment.

Links

PubMed PMC235991

Keywords

Bacteriological Techniques; Cell Division; Deoxyribonucleases; Mycoplasma/growth & development; Mycoplasma/isolation & purification; Mycoplasma/ultrastructure; Pronase; Sonication; Trypsin

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).

Phenotype Annotations

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<protect>

Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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Notes

References

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