Difference between revisions of "PMID:4904235"
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| + | {| id="I505ec7a0d65dd" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Held, WA and Smith, OH ''' (1970) Mechanism of 3-methylanthranilic acid derepression of the tryptophan operon in Escherichia coli. ''J. Bacteriol.'' '''101''':209-17 | ||
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| + | !align=left |Abstract | ||
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| + | 3-Methylanthranilic acid (3MA) inhibits growth and causes derepression of the tryptophan biosynthetic enzymes in wild-type strains of Escherichia coli. Previous reports attributed this effect to an inhibition of the conversion of 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate to indole-3-glycerol phosphate and a consequent reduction in the concentration of endogenous tryptophan. Our studies have shown that 3MA-resistant mutants linked to the tryptophan operon have a feedback-resistant anthranilate synthetase; mutants with an altered indole-3-glycerol phosphate synthetase were not found. 3MA or 7-methylindole can be metabolized to 7-methyltryptophan, and 3MA, 7-methylindole, and 7-methyltryptophan lead to derepression of the tryptophan operon. Furthermore, 3MA-resistant mutants are also resistant to 7-methylindole derepression. These results strongly suggest that the primary cause of derepression by 3MA is through its conversion to 7-methyltryptophan, which can inhibit anthranilate synthetase, thereby decreasing the concentration of endogenous tryptophan. Unlike 5- or 6-methyltryptophan, 7-methyltryptophan does not appear to function as an active corepressor. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=4904235 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC250472 PMC250472] | ||
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| + | !align=left |Keywords | ||
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| + | Anthranilic Acids/analysis; Anthranilic Acids/metabolism; Culture Media; Enzyme Repression/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Glycerol/metabolism; Indoles/metabolism; Kinetics; Ligases; Molecular Biology; Mutation; Operon; Pentoses/metabolism; Phosphates/metabolism; Tritium; Tryptophan/biosynthesis; Tryptophan/metabolism | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
| + | {{LitMaterials}} | ||
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| + | ==Phenotype Annotations== | ||
| + | {{AnnotationTableHelp}} | ||
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| + | {| id="I505ec7a0d940b" class=" tableEdit Phenotype_Table_2" | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 03:26, 23 September 2012
| Citation |
Held, WA and Smith, OH (1970) Mechanism of 3-methylanthranilic acid derepression of the tryptophan operon in Escherichia coli. J. Bacteriol. 101:209-17 |
|---|---|
| Abstract |
3-Methylanthranilic acid (3MA) inhibits growth and causes derepression of the tryptophan biosynthetic enzymes in wild-type strains of Escherichia coli. Previous reports attributed this effect to an inhibition of the conversion of 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate to indole-3-glycerol phosphate and a consequent reduction in the concentration of endogenous tryptophan. Our studies have shown that 3MA-resistant mutants linked to the tryptophan operon have a feedback-resistant anthranilate synthetase; mutants with an altered indole-3-glycerol phosphate synthetase were not found. 3MA or 7-methylindole can be metabolized to 7-methyltryptophan, and 3MA, 7-methylindole, and 7-methyltryptophan lead to derepression of the tryptophan operon. Furthermore, 3MA-resistant mutants are also resistant to 7-methylindole derepression. These results strongly suggest that the primary cause of derepression by 3MA is through its conversion to 7-methyltryptophan, which can inhibit anthranilate synthetase, thereby decreasing the concentration of endogenous tryptophan. Unlike 5- or 6-methyltryptophan, 7-methyltryptophan does not appear to function as an active corepressor. |
| Links | |
| Keywords |
Anthranilic Acids/analysis; Anthranilic Acids/metabolism; Culture Media; Enzyme Repression/drug effects; Escherichia coli/enzymology; Escherichia coli/growth & development; Glycerol/metabolism; Indoles/metabolism; Kinetics; Ligases; Molecular Biology; Mutation; Operon; Pentoses/metabolism; Phosphates/metabolism; Tritium; Tryptophan/biosynthesis; Tryptophan/metabolism |
| edit table |
Main Points of the Paper
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Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
Phenotype Annotations
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<protect>
| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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</protect>
Notes
References
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