Difference between revisions of "PMID:7009581"

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{|  id="X5064d9f241af7"  class=" tableEdit PMID_info_table" 
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'''Lazzaroni, JC  and Portalier, RC '''  (1981) Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. ''J. Bacteriol.'' '''145''':1351-8
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!align=left  |Abstract
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Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.
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[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7009581 PubMed] [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217139 PMC217139]
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!align=left  |Keywords
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Alkaline Phosphatase/metabolism; Bacterial Proteins/metabolism; Cell Membrane/physiology; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Genes; Membrane Proteins/metabolism; Mutation
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==Main Points of the Paper ==
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== Materials and Methods Used ==
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==Phenotype Annotations==
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==Notes==
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==References==
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[[Category:Publication]]

Revision as of 17:57, 27 September 2012

Citation

Lazzaroni, JC and Portalier, RC (1981) Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. J. Bacteriol. 145:1351-8

Abstract

Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.

Links

PubMed PMC217139

Keywords

Alkaline Phosphatase/metabolism; Bacterial Proteins/metabolism; Cell Membrane/physiology; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Genes; Membrane Proteins/metabolism; Mutation

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Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

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Phenotype Annotations

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Phenotype of Taxon Information Genotype Information (if known) Condition Information OMP ID OMP Term Name ECO ID ECO Term Name Notes Status
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</protect>

Notes

References

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