PMID:7009581

Citation	Lazzaroni, JC and Portalier, RC (1981) Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12. J. Bacteriol. 145:1351-8
Abstract	Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.
Links	PubMed PMC217139
Keywords	Alkaline Phosphatase/metabolism; Bacterial Proteins/metabolism; Cell Membrane/physiology; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli/physiology; Genes; Membrane Proteins/metabolism; Mutation
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Materials and Methods Used

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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Gal5 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolPAB+ Genotype of Experimental Strain : tolPAB- in 207	Reference Condition:	0000085	cell envelope phenotype	0000181	in vitro assay evidence	A mutation in the strain 207's tolPAB operon caused the exretion of alakaline phosphate, RNAase I and DNAse I.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Gal5 NCBI Taxon ID: 83333	Genotype of Reference Strain: phosT+ Genotype of Experimental Strain : phosT- in 188	Reference Condition:			0000181	in vitro assay evidence	Mutation the phosT gene caused resistance to arsenate resistance.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: Gal5 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolPAB+ Genotype of Experimental Strain : tolPAB- in 236	Reference Condition:	0000085	cell envelope phenotype	0000181	in vitro assay evidence	A mutation in the strain 236's tolPAB operon caused the exretion of alakaline phosphate, RNAase I and DNAse I.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 207 NCBI Taxon ID: 83333	Genotype of Reference Strain: phosT+ Genotype of Experimental Strain : phosT- in 207c	Reference Condition:			0000181	in vitro assay evidence	Mutation the phosT gene caused resistance to arsenate resistance.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 188 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolPAB+ Genotype of Experimental Strain : tolPAB- in 236c	Reference Condition:			0000181	in vitro assay evidence	Mutation the tolPAB gene caused increased activity of alkaline phosphatase. See Figure 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 188 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolPAB+ Genotype of Experimental Strain : tolPAB- in 207c	Reference Condition:			0000181	in vitro assay evidence	Mutation the tolPAB gene caused increased activity of alkaline phosphatase. See Figure 1.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 236 NCBI Taxon ID: 83333	Genotype of Reference Strain: phosT+ Genotype of Experimental Strain : phosT- in 236c	Reference Condition:			0000181	in vitro assay evidence	Mutation the phosT gene caused resistance to arsenate resistance.
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: 188 NCBI Taxon ID: 83333	Genotype of Reference Strain: tolPAB+ Genotype of Experimental Strain : tolPAB- in 207c	Reference Condition:			0000181	in vitro assay evidence	The distribution of alkaline phosphatase activity within perplasmic leaky mutants showed that the remaining intracellular enzyme could be released by osmotic shock.
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References

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