Difference between revisions of "PMID:3020376"
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| + | {| id="Y508ac28956ccf" class=" tableEdit PMID_info_table" | ||
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| + | !align=left |Citation | ||
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| + | '''Yamagishi, J , Yoshida, H , Yamayoshi, M and Nakamura, S ''' (1986) Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli. ''Mol. Gen. Genet.'' '''204''':367-73 | ||
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| + | !align=left |Abstract | ||
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| + | DNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the first base of the 426th codon of the gyrB gene, resulting in an amino acid change from aspartic acid to asparagine, and nal-31 was an A to G transition at the first base of the 447th codon, resulting in an amino acid change from lysine to glutamic acid. This indicates tha mutations in the gyrB gene are responsible for nalidixic acid resistance. | ||
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| + | !align=left |Links | ||
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| + | [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3020376 PubMed] | ||
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| + | !align=left |Keywords | ||
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| + | Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; DNA Topoisomerases, Type II/genetics; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Mutation; Nalidixic Acid/pharmacology; Plasmids | ||
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| + | ==Main Points of the Paper == | ||
| + | {{LitSignificance}} | ||
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| + | == Materials and Methods Used == | ||
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| + | ==Phenotype Annotations== | ||
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| + | !|Phenotype of!!Taxon Information!!Genotype Information (if known)!!Condition Information!!OMP ID!!OMP Term Name!!ECO ID!!ECO Term Name!!Notes!!Status | ||
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| + | ==Notes== | ||
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| + | ==References== | ||
| + | {{RefHelp}} | ||
| + | <references/> | ||
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| + | [[Category:Publication]] | ||
Revision as of 12:04, 26 October 2012
| Citation |
Yamagishi, J , Yoshida, H , Yamayoshi, M and Nakamura, S (1986) Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli. Mol. Gen. Genet. 204:367-73 |
|---|---|
| Abstract |
DNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the first base of the 426th codon of the gyrB gene, resulting in an amino acid change from aspartic acid to asparagine, and nal-31 was an A to G transition at the first base of the 447th codon, resulting in an amino acid change from lysine to glutamic acid. This indicates tha mutations in the gyrB gene are responsible for nalidixic acid resistance. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA Restriction Enzymes; DNA Topoisomerases, Type II/genetics; Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Mutation; Nalidixic Acid/pharmacology; Plasmids |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Notes
References
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