Plate bioassay for antimicrobial activity

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Purpose

This assay can be used to test for production of or sensitivity to antimicrobial agents by measuring a zone of inhibition around an antimicrobial compound.

Materials Needed

(For testing four plates)

8 NBY Agar plates

25-30 mL NBY Top Agar

750 mL water

Sterile toothpicks

Millimeter measuring stick

Indicator Fungus-Geotrichum candidum

30 C incubator

65 C incubator

Papers where this or a similar method has been used

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References

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