PMID:21453489

Citation	Poteete, AR (2011) Recombination phenotypes of Escherichia coli greA mutants.BMC Mol. Biol. 12:12
Abstract	The elongation factor GreA binds to RNA polymerase and modulates transcriptional pausing. Some recent research suggests that the primary role of GreA may not be to regulate gene expression, but rather, to promote the progression of replication forks which collide with RNA polymerase, and which might otherwise collapse. Replication fork collapse is known to generate dsDNA breaks, which can be recombinogenic. It follows that GreA malfunction could have consequences affecting homologous recombination.
Links	PubMed Online version:10.1186/1471-2199-12-12
Keywords	Escherichia coli; Escherichia coli Proteins; Mutation; Phenotype; Rec A Recombinases; Recombination, Genetic; Transcription Factors
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 8333	Genotype of Reference Strain: greA+ Genotype of Experimental Strain : greA-D41A	Reference Condition:	OMP:0005050	decreased frequency of homologous recombination			Figure 1 and Figure 2: decreased lambda Red-mediated recombination
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 8333	Genotype of Reference Strain: greA+ Genotype of Experimental Strain : greA-D41N	Reference Condition:	OMP:0005050	decreased frequency of homologous recombination			Figure 1 and Figure 2: decreased lambda Red-mediated recombination
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 8333	Genotype of Reference Strain: greA+ Genotype of Experimental Strain : greA-E44K	Reference Condition:	OMP:0005050	decreased frequency of homologous recombination			Figure 1 and Figure 2: decreased lambda Red-mediated recombination
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: MG1655 NCBI Taxon ID: 8333	Genotype of Reference Strain: greA+ Genotype of Experimental Strain : greA(del)	Reference Condition:	OMP:0005050	decreased frequency of homologous recombination			Figure 1 and Figure 2: decreased lambda Red-mediated recombination
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