PMID:4882748

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Citation

Carter, JR, Fox, CF and Kennedy, EP (1968) Interaction of sugars with the membrane protein component of the lactose transport system of Escherichia coli.Proc. Natl. Acad. Sci. U.S.A. 60:725-32

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Bacterial Proteins; Binding Sites; Biological Transport, Active; Carbohydrates; Carbon Isotopes; Cell Membrane; Cysteine; Escherichia coli; Ethylmaleimide; Formaldehyde; Galactose; Glycosides; Lactose

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

  • Parent Strain: ML308 (i-z+y+a+)
  • Membrane vesicle isolation
  • TDG protection
  • NEM labelling of LacY

Phenotype Annotations

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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

Escherichia coli

NCBI:562

ML308-225

lacZ

beta-galactosidase negative

Metabolic Activity

Biochemical Assay

LacZ assay

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Notes