PMID:4933690

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Citation

Wilson, TH, Kusch, M and Kashket, ER (1970) A mutant in Escherichia coli energy-uncoupled for lactose transport: a defect in the lactose-operon.Biochem. Biophys. Res. Commun. 40:1409-14

Abstract

No abstract in PubMed

Links

PubMed

Keywords

Biological Transport, Active; Carbon Isotopes; Cell Membrane; Coliphages; Escherichia coli; Galactose; Glycosides; Lactose; Molecular Biology; Mutation; Nitrobenzenes; Operon; Transduction, Genetic

Main Points of the Paper

Please summarize the main points of the paper.

Materials and Methods Used

  • Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-, F-)
  • Additional Strain: X71-54
  • Additional Strain: Ca77 (lactose-deleted, B1-, Hfr)
  • β-galactosidase assay
  • ONPG uptake assay
  • Thiomethylgalactoside (TMG) transport assay
  • P1 Transduction

Phenotype Annotations

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Species Taxon ID Strain Gene (if known) OMP Phenotype Details Evidence Notes

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Notes