PMID:4583216

Citation	Ricard, M and Hirota, Y (1973) Process of cellular division in Escherichia coli: physiological study on thermosensitive mutants defective in cell division.J. Bacteriol. 116:314-22
Abstract	Thermosensitive fts mutants of Escherichia coli belonging to seven previously identified genetic classes (ftsA to ftsG) were studied from a physiological standpoint. These mutants immediately stopped dividing and formed multinucleated filaments when the temperature was shifted to 41 C. Macromolecular syntheses (deoxyribonucleic acid), ribonucleic acid, cell mass, and murein) continued exponentially for at least 40 to 120 min. The number of surviving bacteria remained constant during the time of incubation, and this number began to decrease exponentially, as the rate of cell mass increase leveled off from the initial rate. The recovery of cell division at 30 C in these filamentous cells was studied after 60 min of incubation at 41 C. The existence of three types of mutants was shown. The ftsA and ftsE mutants resumed cell division without new protein synthesis; ftsD mutants resumed cell division only if new protein synthesis occured, while ftsB, C, F and G mutants did not resume cell division at all. No alteration in the cell envelope was detected by the method used here, although the ftsA, B, D, F and G mutations, in contrast with ftsC and E, caused an increased resistance to penicillin G. It was also shown that the recA mutation did not suppress the effect of the fts mutations and that none of the lysogenic fts mutants induced prophage multiplication while forming filaments. The effects of osmotic pressure and salts which rescue the mutant phenotype is described.
Links	PubMed
Keywords	Bacterial Proteins; Carbon Radioisotopes; Cell Division; Cell Wall; DNA, Bacterial; Escherichia coli; Fluorescence; Lysogeny; Mutation; Penicillin G; Penicillin Resistance; Peptidoglycan; RNA, Bacterial; Temperature; Thymidine; Tritium; Uracil
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Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli		MFT108	ftsC	abolished septation at high temperature temperature-sensitive increased filamentation	Morphology	septation arrested immediately when the temperature of incubation was shifted from 30C to 41C	Microscopy
Escherichia coli		PAT84	ftsA	decreased survival when grown at high temperature temperature-sensitive decreased survival	Growth	CFU counts decrease when cells are grown at 41C	Plating Assay	Figure 1A
Escherichia coli		MFT84	ftsB	decreased survival when grown at high temperature temperature-sensitive decreased survival	Growth	CFU counts decrease when cells are grown at 41C	Plating Assay	Figure 1B
Escherichia coli		PAT84	ftsA	abolished septation at high temperature temperature-sensitive increased filamentation	Morphology	septation arrested immediately when the temperature of incubation was shifted from 30C to 41C	Microscopy
Escherichia coli		MFT84	ftsB	abolished septation at high temperature temperature-sensitive increased filamentation	Morphology	septation arrested immediately when the temperature of incubation was shifted from 30C to 41C	Microscopy
Escherichia coli		MFT108	ftsC	increased production of anucleate cells at high temperature temperature-sensitive increase of anucleate cells	Morphology	residual septation occurring at the ends of the filaments	Microscopy
Escherichia coli		MFT84	ftsB	increased production of anucleate cells at high temperature temperature-sensitive increase of anucleate cells	Morphology	residual septation occurring at the ends of the filaments	Microscopy
Escherichia coli		MFT108	ftsC	decreased rate of DNA synthesis at high temperature temperature-sensitive decrease in DNA synthesis	Other	3H-thymidine incorporation	Biochemical Assay
Escherichia coli		MFT103	ftsD	decreased rate DNA synthesis at high temperature temperature-sensitive decrease in DNA synthesis	Other	3H-thymidine incorporation-decreased rate begins ~90 minutes after temperature shift	Biochemical Assay	Figure 2 top
Escherichia coli		MFT103	ftsD	decreased rate of RNA synthesis at high temperature temperature-sensitive decrease in RNA synthesis	Other	14C-uracil incorporation-decreased rate begins 40 minutes after temperature shift	Biochemical Assay	Figure 2 bottom
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References

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