PMID:1100857
| Citation |
Aswad, D and Koshland, DE (1975) Isolation, characterization and complementation of Salmonella typhimurium chemotaxis mutants.J. Mol. Biol. 97:225-35 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Chemotaxis; Genes, Dominant; Genes, Recessive; Genetic Complementation Test; Genotype; Mutation; Recombination, Genetic; Salmonella typhimurium; Species Specificity |
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Main Points of the Paper
Please summarize the main points of the paper.
- Isolation of non-chemotactic mutants
- Description of the preformed liquid gradient technique
- utilizes a preformed gradient in a vertical column of liquid medium
- separates wild-type, non-motile, and non-motile but chemotactic into 3 regions
- None of the 72 mutants isolated exhibited a frequency of spontaneous tumbling
- 68 exhibited smooth co-ordinated motility with no evidence of tumbling
- 4 exhibited uncoordinated motility
- Results suggest there may be as many as 6 genetically distinct classes of che mutants in Salmonella
Materials and Methods Used
Please list the materials and methods used in this paper (strains, plasmids, antibodies, etc).
- For the isolation of non-chemotactic mutants
- Diethylsulfate mutagenesis
- negative swarm selection on Tryptone semi-solid medium (0.3% agar)
- preformed liquid gradient technique (described in detail)
- For complementations and strain constructions
- Alternative method of abortive transduction
- Strains
- all strains were derived from Salmonella typhimurium LT2
Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
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