PMID:14168696
| Citation |
BECKWITH, JR (1964) A DELETION ANALYSIS OF THE LAC OPERATOR REGION IN ESCHERICHIA COLI.J. Mol. Biol. 8:427-30 |
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| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Deletion; Lac; Operator; Escherichia coli; Galactosidases; Genetics; Membrane Transport Proteins; Mutation; RNA, Bacterial; Research; Enzymes; Revertants; Constitutive |
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Main Points of the Paper
- The lac operator does not lie in the same region in which oo mutants are found.
Materials and Methods Used
- Mutant Classes
- oc mutants
- lost the sensitivity to repressor, therefore expressing β-galactosidase constitutively in the presence of the repressor
- very closely linked to z- mutations mapped at the beginning of the z gene
- oo mutants
- shuts off the production of all 3 enzymic activities of the lac operon
- oc mutants
- Selection of z-y+ revertants on melibiose-agar
Phenotype Annotations
<protect>
| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
oc |
constitutive expression of β-galactosidase in the presence of repressor |
Metabolic Activity |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo |
constitutive repression of all 3 enzymic activities of the lac operon |
Metabolic Activity |
Biochemical Assay |
|||
|
Escherichia coli |
NCBI:562 |
oo2-M116 |
loss of repressibility |
Metabolic Activity |
Biochemical Assay |
Table 1 | ||
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</protect>
