PMID:4577939
| Citation |
Kusch, M and Wilson, TH (1973) Defective lactose utilization by a mutant of Escherichia coli energy-uncoupled for lactose transport. The advantages of active transport versus facilitated diffusion.Biochim. Biophys. Acta 311:109-22 |
|---|---|
| Abstract |
No abstract in PubMed |
| Links | |
| Keywords |
Biological Transport; Biological Transport, Active; Carbon Isotopes; Cell Division; Densitometry; Diffusion; Disaccharides; Enzyme Induction; Escherichia coli; Galactosidases; Kinetics; Lactose; Mutation; Transduction, Genetic |
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Main Points of the Paper
Please summarize the main points of the paper.
Materials and Methods Used
- Parent Strain: X71 (i-z+y+a-, proC-, try-, B1-)
- Additional Strain: X71-54 (i-z+yUNa-)- energy uncoupled mutant
- Additional Strain: X71-15 (i+z+y+a-)
- Additional Strain: X9003 (i+z-y+a+, B1-)
- Additional Strain: 54-41 (i+z+yUNa-)
- Additional Strain: 316 (i-z+y-a-)- y- strain
- β-galactosidase assay
- α-galactosidase assay
- Carrier-mediated transport assay
- thiogalactoside transacetylase activity was determined
- P1 Transduction
Phenotype Annotations
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| Species | Taxon ID | Strain | Gene (if known) | OMP | Phenotype | Details | Evidence | Notes |
|---|---|---|---|---|---|---|---|---|
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
inability to grow on lactose |
Growth |
in a minimal salts medium |
Other |
in text, pg. 113 |
|
Escherichia coli |
NCBI:562 |
316 |
lacY- |
no induction of alpha-galactosidase |
Metabolic Activity |
alpha-gal activity |
Biochemical Assay |
in text- pg. 116 |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
White colonies on MacConkey melibiose indicator plates |
Growth |
inability to ferment 0.2% melibiose |
Plating Assay |
Table 4 |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
reduced intracellular IPTG accumulation |
Other |
mutant reached a maximum intracellular concentration of 0.1 mM compared to 0.4 mM IPTG for the energy-coupled parent |
Other |
Figure 1- [14C]IPTG uptake |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
reduced rate of beta-galactosidase induction |
Metabolic Activity |
Biochemical Assay |
Figure 4- beta-galactosidase activity measured after lactose induction | |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
reduced growth rate on melibiose |
Growth |
in a minimal salts medium |
Plating Assay |
Table 3 |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
reduced rate of alpha-galactosidase induction |
Metabolic Activity |
Biochemical Assay |
Figure 5- alpha-galactosidase activity measured after melibiose induction | |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
decreased affinity for lactose |
Other |
Other |
Table 5- kinetics of transporter for potential inducer molecules | |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
Light pink colonies on MacConkey lactose indicator plates |
Growth |
reduced ability to ferment 0.2% lactose (fully restored when IPTG is present) |
Plating Assay |
Table 4 |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
Light pink colonies on MacConkey melibiose indicator plates |
Growth |
inability to ferment 0.2% melibiose |
Plating Assay |
Table 4 |
|
Escherichia coli |
NCBI:562 |
X71-54 |
lacYUN |
Light pink colonies on MacConkey melibiose indicator plates |
Growth |
reduced ability to ferment 0.2% melibiose |
Plating Assay |
Table 4 |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
no growth on lactose |
Growth |
in liquid culture with lactose concentrations b/t 5M and 50mM |
Plating Assay |
in text, pg. 117- addition of 0.5mM IPTG added to lactose medium lead to growth comparable to parent with no constitutive cells |
|
Escherichia coli |
NCBI:562 |
54-41 |
lacYUN |
increased affinity for melibiose |
Other |
Other |
Table 5- kinetics of transporter for potential inducer molecules | |
| edit table |
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