PMID:794878
| Citation |
Gellert, M, O'Dea, MH, Itoh, T and Tomizawa, J (1976) Novobiocin and coumermycin inhibit DNA supercoiling catalyzed by DNA gyrase.Proc. Natl. Acad. Sci. U.S.A. 73:4474-8 |
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| Abstract |
Novobiocin and coumermycin are known to inhibit the replication of DNA iing of DNA catalyzed by E. coli DNA gyrase, a recently discovered enzyme that introduces negative superhelical turns into covalently circular DNA. The activity of DNA gyrase purified from a coumermycin-resistant mutant strain is resistant to both drugs. The inhibition by novobiocin of colicin E1 plasmid DNA replication in a cell-free system is partially relieved by adding resistant DNA gyrase. Both in the case of coliclls. DNA molecules which are converted to the covalently circular form in thepresence of coumermycin remain relaxed, instead of achieving their normal supercoiled conformation. We conclude that DNA gyrase controls the supercoiling of DNA in E. coli. |
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| Keywords |
Anti-Bacterial Agents; Colicins; Coumarins; DNA Replication; DNA, Bacterial; DNA, Circular; DNA, Viral; Escherichia coli; Mutation; Novobiocin; Nucleic Acid Conformation; Plasmids; Pyrroles |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
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Novobiocin and coumermycin inhibit DNA replication in E-coli. DNA gyrase relieves any strain caused by the unwinding of the DNA, therefore preventing DNA to supercoil during replication. |
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DNA gyrase is resistant to both Novobiocin and coumermycin. In the toluenized cells of E.coli the drug inhibits the synthesis of DNA but not repair synthesis. |
Coumermycin-resistant E.coli mutants. Starting strain NT525, similar to strain K12. NI708, NI741, CRT46 dnaA, NI748, N1071, YS1 |
DNA gyrase was isolated from N99 recB21 |
Strain Nt525 resistant to Novobiocin grew on tryptone agar of 600 ug/ml of Novobiocin. Coumermycin resistant isolates: NI741 and NI748 grew well in a liquid culture of 60ug/ml of coumermycin. Growth of other starins were blocked by 15ug/ml of coumermycin. Relaxed and intracellulary supercoiled samples were prepared. Relaxed and circular phage DNA was also prepared. |
Mutants of NI708 were highly resistant novobiocin drug. NI748 used for the purification DNA gyrase. |
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Notes
References
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