PMID:2553680
| Citation |
Braun, V (1989) The structurally related exbB and tolQ genes are interchangeable in conferring tonB-dependent colicin, bacteriophage, and albomycin sensitivity. J. Bacteriol. 171:6387-90 |
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| Abstract |
Double exbB tolQ mutants of Escherichia coli were completely resistant to bacteriophages T1 and phi 80, in contrast to strains with exbB or tolQ mutations, which were sensitive. Cells carrying mutations in exbB were partially tolerant to colicins B, D, and M and became fully tolerant by the introduction of tolQ mutations. This suggested involvement of both exbB and tolQ in tonB-dependent uptake. |
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| Keywords |
Anti-Bacterial Agents/pharmacology; Bacterial Proteins/genetics; Colicins/pharmacology; Coliphages/genetics; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Escherichia coli Proteins; Ferrichrome/pharmacology; Genes, Bacterial; Mutation; T-Phages/genetics |
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Main Points of the Paper
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Materials and Methods Used
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Phenotype Annotations
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| Phenotype of | Taxon Information | Genotype Information (if known) | Condition Information | OMP ID | OMP Term Name | ECO ID | ECO Term Name | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|
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a mutation or genetic difference within a strain |
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0000276 |
increased resistance to anitbiotics |
0000181 |
in vitro assay evidence |
Mutations to the exbB and tolQ genes caused resistance to T1 Phage. |
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a mutation or genetic difference within a strain |
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0006027 |
increased resistance to bacteriocin |
0000181 |
in vitro assay evidence |
Insertion of transposon into the exbB gene caused increased tolerance toward Colicin B and albomycin. See Table 2 for data. |
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a mutation or genetic difference within a strain |
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|
0000276 |
increased resistance to anitbiotics |
0000181 |
in vitro assay evidence |
The exbB, tolQ double mutation in strain HE2 conferred tolerance to all the colicins and phages tested as well as Albomycin. See Table 2 for full data. |
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Notes
The lack of exbB and tolQ encoded functions were negated by the overproduction of the TonB protein required for T1 sensitivity as seen in Table 2, implying with only tonB T1 sensitivity can be conferred.
References
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