PMID:6450313

Citation	Lengeler, J (1980) Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin.Mol. Gen. Genet. 179:49-54
Abstract	From cultures of sensitive bacteria, treated with the antibiotic streptozotocin, two classes of resistant mutants can be isolated: 1) mutants, resistant under all the conditions tested to even the highest doses of the antibiotic. These are either pleiotropic-defective, pts-mutants, or more frequently, mutants lacking a transport system (enzyme IINag-complex of the PEP-dependent phosphotransferase system) encoded by the gene nagE. This gene is inducible by N-acetyl-glucosamine and seems to be part of the nag operon. The transport system in question is responsible for the uptake of N-acetyl-glucosamine, of D-glucosamine and of streptozotocin; 2) conditional resistant mutants which are unable to energize or to synthesize the streptozotocin transport system under certain growth conditions but do have the transport activity under other conditions. These include a) mutants auxotrophic for amino acids, vitamins, or nucleotides, b) mutants negative or sensitive to carbohydrates in the medium, and c) mutants wth defects in energy metabolism such as PEP synthesis.
Links	PubMed
Keywords	Dose-Response Relationship, Drug; Drug Resistance, Microbial; Escherichia coli; Mutation; Phenotype; Streptozocin
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Phenotype Annotations

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Phenotype of	Taxon Information	Genotype Information (if known)	Condition Information	OMP ID	OMP Term Name	ECO ID	ECO Term Name	Notes	Status
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: CA8445 NCBI Taxon ID: 83333	Genotype of Reference Strain: cya crp Genotype of Experimental Strain : cya (del) crp (del)	Reference Condition:	OMP:0006057	decreased resistance to aminoglycoside	ECO:0000178	in vivo assay data	sensitivity to streptozotocin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: L176 NCBI Taxon ID: 83333	Genotype of Reference Strain: cyaA Genotype of Experimental Strain : cyaA in strain L176	Reference Condition:	OMP:000605	decreased resistance to aminoglycoside	ECO:0000178	in vivo assay data	sensitivity to streptozotocin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: LR2-163, LR-12, LR-29 NCBI Taxon ID: 83333	Genotype of Reference Strain: nagE Genotype of Experimental Strain : nagE in strain LR2-163, LR-12, LR-29	Reference Condition:	OMP:0006057	decreased resistance to aminoglycoside	ECO:0000178	in vivo assay data	Resistance to Streptozotocin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: LR2-163, LR-12, LR-29	Genotype of Reference Strain: nagE Genotype of Experimental Strain : nagE in strain LR2-163, LR-12, LR-29	Reference Condition:			ECO:0000178	in vivo assay data	Sensitive to D-mannose
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: IR-101	Genotype of Reference Strain: manA Genotype of Experimental Strain : manA -	Reference Condition:	OMP:0006057	deceased resistance to aminoglycoside	ECO:0000178	in vivo assay data	sensitive to Streptozotocin
a mutation or genetic difference within a strain	Taxon: Escherichia coli Strain: K-12 Substrain: NCBI Taxon ID: 83333	Genotype of Reference Strain: pfkA Genotype of Experimental Strain : pfkA	Reference Condition:	OMP:0006058	increased resistance to aminoglycoside	ECO:0000182	increased resistance to antibiotics	Resistant to streptozotocin
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