PMID:4869617

Citation	King, S and Metzger, WI (1968) A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar.Appl Microbiol 16:577-8
Abstract	A new agar plating medium for the isolation of enteric pathogens is described. This medium contains greater quantities of peptone to offset the inhibitory effects of bile salts. Additional carbohydrates and larger quantities of those previously used have also been incorporated into the medium to differentiate pathogens from some of the slow lactose fermenters. The use of an indicator system never used before in a differential plating medium opens new possibilities for improvement over traditional and newer selective media. Known strains from the various genera of Enterobacteriaceae have been tested for colonial differentiation as well as for possible inhibitory effects. The results show the medium to be highly selective for the shigellae as well as for other enteric pathogens.
Links	PubMed
Keywords	Agar; Bile Acids and Salts; Enterobacter; Enterobacteriaceae; Escherichia coli; Indicators and Reagents; Proteus; Salmonella; Shigella
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Main Points of the Paper

• Describes the development of Hektoen Agar (HE)
  • selective for Shigella as well as other pathogens while inhibiting normal intestinal flora
  • contains several indicators which allow for colonies to be specifically identified, increasing the confidence of the identification
• Part II

Materials and Methods Used

• Medium contains:
  • Lactose
  • Sucrose
  • Salicin
  • Bile salts
  • Sodium chloride
  • Proteose peptone
  • Beef extract
  • Agar
  • Bromothymol blue
  • Andrades' indicator

• Post-Boiling Additives
  • Sodium thiosulfate
  • Ferric ammonium citrate
  • Sodium deoxycholate
  • H₂S indicator

• Strains
  • Escherichia coli
  • Citrobacter
  • Proteus mirabilis
  • Paracolobactrum arizonae
  • Salmonella
  • Shigella
  • Pseudomonas
  • Providencia

Phenotype Annotations

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Species	Taxon ID	Strain	Gene (if known)	OMP	Phenotype	Details	Evidence	Notes
Escherichia coli	NCBI:562			No growth	Growth	on Hektoen Agar	Plating Assay	Table 1- non-pathogenic enteric bacteria
Citrobacter	NCBI:544			Slow growth on Hektoen Agar	Growth	Non-pathogenic enteric bacteria	Plating Assay	Table 1
Proteus mirabilis	NCBI:584			No growth	Growth	on Hektoen Agar	Plating Assay	Table 1- non-pathogenic enteric bacteria
Paracolobactrum arizonae	NCBI:59203			Growth	Growth	on Hektoen Agar	Plating Assay	Table 1
Salmonella	NCBI:590			Growth	Growth	on Hektoen Agar	Plating Assay	Table 1
Shigella	NCBI:620			Growth	Growth	on Hektoen Agar	Plating Assay	Table 1
Shigella	NCBI:620			Green colonies	Staining Results	growth on Hektoen agar	Plating Assay	Table 2-strain doesn't utilize any of the sugars present in the media.
Shigella	NCBI:620			Raised colonies	Morphology	growth on Hektoen agar	Plating Assay	Table 2
Shigella	NCBI:620			Moist colonies	Morphology	growth on Hektoen agar	Plating Assay	Table 2
Salmonella	NCBI:590			Blue-green to blue colonies with black centers	Growth	on Hektoen Agar	Plating Assay	Table 2- strain unable to utilize sugars present in the media
Salmonella	NCBI:590			Blue-green to blue colonies with black centers	Growth	tested on Hektoen Agar	Plating Assay	Table 2- strain unable to utilize sugars present in the media
Pseudomonas	NCBI:286			Green colonies	Staining Results	on Hektoen Agar	Plating Assay	Table 2- strain cannot utilize sugars present in the medium
Pseudomonas	NCBI:286			Brownish colonies	Staining Results	on Hektoen Agar	Plating Assay	Table 2- strain cannot utilize sugars present in the medium
Pseudomonas	NCBI:286			Flat colonies	Morphology	on Hektoen Agar	Plating Assay	Table 2- strain cannot utilize sugars present in the medium
Pseudomonas	NCBI:286			Irregular colonies	Morphology	on Hektoen Agar	Plating Assay	Table 2- strain cannot utilize sugars present in the medium
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References

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