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id: ECO:0000239
name: 3C-seq evidence
def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing." [ECO:MCC, PMID:23411633]
synonym: "3C sequencing" EXACT []
synonym: "chromosome conformation capture sequencing evidence" RELATED []
synonym: "circularized 3C sequencing" EXACT []
synonym: "circularized 3C-seq" EXACT []
is_a: ECO:0000232 ! chromosome conformation-based evidence
comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation.
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