id: ECO:0000230
name: chromatin immunoprecipitation-chip evidence
def: "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927]
synonym: "ChIP-chip evidence" EXACT []
synonym: "ChIP-on-chip evidence" EXACT []
xref: [ MI:0225 "chromatin immunoprecipitation array"]
is_a: ECO:0000226 ! chromatin immunoprecipitation evidence
relationship: IAO:0000136: GO:0043565 ! is about sequence-specific DNA binding
relationship: OBI:0000312: OBI:0001248 ! is_specified_output_of ChIP-chip assay
comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp.
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