id: ECO:0000110
name: RNA protection assay evidence
def: "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." [ECO:SW, PMID:23457339, TAIR:TED]
synonym: "IEP: RNA protection assay" RELATED []
synonym: "ribonuclease protection assay" EXACT []
synonym: "RNAse protection assay" RELATED []
synonym: "RPA" NARROW []
is_a: ECO:0001106 ! nuclease protection assay evidence
relationship: IAO:0000136: GO:0010467 ! is about gene expression
relationship: OBI:0000312: OBI:0001670 ! is_specified_output_of RNA protection assay
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